" BRAXY " SHEEP DISEASE IN AUSTRALIA. 607 



except marked muttony odour, muscles dark and haemorrhagic ; no fluids 

 in serous cavities, except pericardium, which is distended with clear effusion ; 

 kidneys and liver congested ; spleen dark, pulpy, but not enlarged ; abo- 

 masum congested and folds oedematous ; heart, subepicardial ecchymoses. 

 Microscopical examination : typical bacilli in muscle and surrounding 

 oedema ; subcutaneous oedema elsewhere, blood, etc., sterile. 



Guineapig 129. — Inoculated with 0-15 cc. 24 hours' old first sub- 

 culture. Animal dead in 20 hours. Post-mortem appearances typical. 



Sheep 19 (5 years old merino ewe). — Inoculated with 0-25 cc. four days' 

 old fourth subculture and Guineapig 134 with 0-1 cc. as control. The 

 following day both animals being normal they were re-inoculated with the 

 same culture as used for guineapig 129 (now eight days old), the same doses 

 being used. The animals both remained normal. Four days later sheep 19 

 was inoculated with 3 cc. seven days' old fourth subculture, and still re- 

 mained normal. Fourteen days later sheep 19 was re-inoculated with 

 0'25 cc. first subculture two days old in ordinary serum broth, and Guineapig 

 134 with OT cc. At the same time Guineapig 143 (not previously inocu- 

 lated) received OT cc. of the same culture. The two guineapigs 

 died in 24 hours with typical post-mortem appearances. Sheep 19 was 

 lame the next day ; temp., 103'4°. The following day the condition was 

 worse ; temp., 104-3°. Death occurred about 56 hours after inoculation. 

 Post-mortem examination : no skin discolouration at region of inoculation ; 

 subcutaneous tissues of inocvilated limb from coronet to pubis infiltrated 

 with effusion clear and semi-gelatinous, unless just around point of inocu- 

 lation ; muscles pale but for one patch, where haemorrhagic. Peritoneal 

 cavity, a small quantity of clear effusion ; spleen pulpy and slightly enlarged 

 liver and kidneys deeply congested ; abomasum deeply congested through- 

 out whole of mucosa, especially at pyloric ring ; submucosa oedematous ; 

 duodenum deeply congested : this gradually gives way to injection of blood 

 vessels ; submucosa very oedematous, and lumen of bowel contains much 

 albuminous oedematous effusion ; pleura, about 6 oz. of clear effusion ; 

 heart normal ; pericardium, little excess of fluid. The typical bacilli were 

 very numerous in the haemorrhagic muscle and the surrounding oedema, 

 but no organisms could be detected in the oedema some distance from the 

 site of inoculation. Spleen and blood apparently sterile. Peritoneal and 

 pleural effusions, a few typical bacilli. Intestinal oedema, many shed 

 epithelial cells and mixed bacteria, very few being of the morphology of the 

 inoculated bacillus. Abomasum mucosa, many bacilli in groups morpho- 

 logically identical with those inoculated. 



Sheep 26 (2 years old). — Inoculated with 5 cc. seven days' old second 

 subculture, Guineapig 148 receiving 1 cc. of same culture. The following 

 day sheep 26 showed a little swelling where inoculated, but there was no 

 lameness ; temperature. 102-6°. The guineapig showed but a slight swelling. 

 Next day both animals were normal. Nineteen days later these animals were 

 re-inoculated with 24 hours' old first culture in ordinary broth — the sheep 

 with 0-25 cc, guineapig 148 with 0-15 cc, guineapig 160 receiving also 0-15 cc. 

 as control. Both guineapigs died within 20 hours. Guineapig 148 showed 

 much subcutaneous oedema, much peritoneal effusion, and numbers of 

 necrosed patches in liver, while guineapig 160 showed comparatively little 

 subcutaneous effusion and no other lesions. The day after inoculation 

 sheep 20 was slightly lame, a little swelling was present, temperature 

 105-5°. The following morning the swelling had increased greatly, the 

 whole of the inner surface of the limb being implicated ; temp., 104-6°. 

 During the day the swelling extended along the abdomen, the animal 

 became dull, the temperature fluctuated, and death occurred 60 hours after 

 inoculation. Post-mortem examination : extensive subcutaneous oedema 

 from coronet to shoulder, no skin discolouration and no crepitation ; small 

 quantity of peritoneal pleural and pericardial effusion ; spleen, kidneys and 

 liver normal ; abomasum, slight congestion but not marked ; lungs oedema- 

 tous ; bronchi and trachea frothy exudate ; heart, ecchymoses in sub- 

 epicardium. Microscopical examination : many typical bacilli present in 



