608 PROCEEDINGS OF SECTION G2. 



oedema and dark muscle tissue of region of inoculation ; mixed organisms 

 in peritoneal effusion ; otherwise organs, etc., apparently sterile. 



Sheep 28 (2 year-old merino). — Inoculated with 5 cc. six days' old first 

 subculture, Gxiineapig 152 receivinsj 1 cc. of same culture. (This culture 

 when 24 hours old was used for guineapig 150 in a dose of 0-075 cc, and 

 death resulted in less than 24 hours, tj-pical lesions being present post-mortem). 

 Guineapig 152 was dead in less than 20 hours. Post-mortem, typical, with 

 no liver necrosis. Sheep 28, lame the following morning, ; subcutaneous 

 tissue of thigh slightly swollen and oedematous ; temp. 103° ; death occurred 

 40 hours after inoculation. Pust-mortem examination : much oedema of 

 subcutaneous tissues from coronet to pubis and along abdomen for several 

 inches ; clear and semi-gelatinous but for a few inches around point of 

 inoculation. Muscles pale, but superficial areas haemorrhagic. Peritoneum : 

 small quantity sero-sanguineous effusion ; vessels injected ; abomasum 

 deeply congested, and patches of submucous haemorrhage near pylorus ; 

 submucosa of folds oedematous. Small intestines diffuse congestion, with 

 some oedematous effusion into lumen ; spleen pulpy but not enlarged ; 

 kidneys and liver slightly congested ; pleural and epicardial cavities contain 

 a small quantity of clear effusion ; limgs enlarged and oedematous ; heart, 

 normal. Microscopical examination showed typical bacilli in haemorrhagic 

 muscle, and in surrounding oedema, but none in clear subcutaneous fluid. 

 Peritoneal fluid : typical bacilli, some long filaments, and tetanus looking 

 sporulating bacilli. Pleural fluid apparently sterile ; blood, a few typical 

 bacilli. Spleen and other organs sterile. 



VI. — Experiments with Clear Sterile CEdema from Sheep. 



Guineapig 40. — Inoculated with O'l cc. subcutaneous oedema from hock 

 of sheep 3, which, kept at 37°C. for two days, remained apparently sterile. 

 The following day a considerable swelling of the inoculated limb was present, 

 extending into the abdominal subcutaneous tissue, but the animal remained 

 bright and ate well. Next day the swelling had decreased markedly. n>e 

 decrease continued until by the third day only a small localised groin nodule 

 was present, which had also disappeared by the fifth day. Fifteen days later 

 this guin apig was inoculated with 0.5 eg. dried muscle of lam.b 1, and death 

 occurred within 28 hours. There was considerable subcutaneous swelling ; 

 internal organs affected with putrefactive changes, examination having been 

 unavoidably delayed. 



Guineapig 41. — Inoculated with 0"1 cc. subcutaneous exudate from 

 shoulder of lamb 4, which kept at 37°C. for 3 days remained apparently sterile. 

 A swelling similar to that noted in guineapig 40 developed rapidly, but the 

 animal remained in good health otherwise and with good appetite. By the 

 fourth day the swelling had disappeared. Fifteen days later, re-inoculated 

 with 0'5 eg. muscle from lamb 1 ; death resulted in less than 20 hours ; post- 

 mortem conditions being similar to those of guineapig 40. 



VII. — Filtered Culture. 



A five days' old culture, showing many bacilli (some degenerating), grown 

 from muscle of sheep 1 and which proved fatal for guineapig 110 in less than 

 20 hours, was filtered through a Chamberland B filter. Of the filtrate 5cc. was 

 injected intraperitoneally into guineapig 112. The animal remained normal. 

 Fourteen days later it received a subcutaneous injection of 0T5 cc. culture 

 as used for sheep 14, guineapig 117 receiving a similar dose as control. Result : 

 both dead within 20 hours. Post-mortem : typical — no liver necrosis in 

 either. 



VIII. — Malignant CEdema and Braxy Experiment. 



Sheep 14 (two years old). — Inoculated with typical culture of malignant 

 oedema bacillus. Animal on following day prostrate, with large emphy- 

 sematous swelling and dark-coloured livid skin at region of inoculation. 



