" BRAXY " SHEEP DISEASE IN AUSTRALIA. 609 



Next day improvement in general condition was observed, but animal was 

 still lame. General recovery occurred later, but for large patch of 

 necrosed and gangrenous tissue in inner surface of thigh. This sloughed, 

 leaving a large wound, which, however, ultimately healed. Thirty-nine days 

 after above inoculation sheep 14 was inoculated in opposite thigh with 0-25 

 cc. first culture from muscle of sheep 1. Guineapigs 112 and 117 were each 

 inoculated with 0T5 cc. of same culture as control. Death occurred in under 

 20 hours ; post-mortem examination typical. 



Sheep 14 the day after second inoculation showed a typical swelling. Tem- 

 perature 103'5. Illness rapidly increased, and death occurred about 30 hours 

 after inoculation. Post-mortem examination : much sero-sanguineous sub- 

 cutaneous effusion extending forward to sternum under skin of abdomen. 

 Muscles pale, with trace? of haemorrhage. Small quantity (few ounces) of peri- 

 toneal and of thoracic effusion. Pericardium distended with clear effusion : 

 abomasum slight congestion of mucosa; no ingesta ; spleen normal; liver 

 and kidneys congested. Microscopical examination showed typical bacilli 

 chiefly in muscles, few in subcutaneous oedema, none in blood, spleen, thoracic 

 fluid, and mixed bacilli in peritoneal fluid. The blood showed no development 

 of bacteria even after prolonged incubation at 37X. 



APPENDIX B. 



Victorian Disease. 



I. — Natural Cases Examined Post-mortem. 



Case I. — Two-year-old wether, fat. — No evidence of struggling. Body 

 cold, except in groms and axilla, and rigor mortis well established (the night 

 had been cold, with slight frost at daybreak). Subcutaneous tissues ; patches 

 of haemorrhage and semi-gelatinous oedema, especially marked around the 

 shoulders and along the neck. Pre-scapular lymph glands swollen, oedematous 

 and haemorrhagic. Abdomen : cavity contains about a pint of somewhat 

 granular sero-sanguineous exudate. Liver mottled with circumscribed, 

 irregular greyish areas of necrosis ; a few distoma present in pile ducts, but 

 no biliary cirrhosis ; lymphatic glands of liver enlarged and oedematous. 

 Kidneys congested. Spleen soft and dark coloured, but not enlarged. 

 Rumen tympanitic. Abomasum : mucous membrane congested ; small 

 quantity of normal ingesta. Small intestines empty ; little ingesta in large 

 intestines, and rectal contents showing flakes of mucoid material. Thorax : 

 8-10 oz. of fluid, clear and but slightly blood-tinged. Lungs : oedematous 

 and more voluminous than normal, but not congested. Mediastinal and 

 bronchial glands enlarged and congested. Pericardium contains 3-4 oz. 

 clear, semi-gelatinous eft'usion. Heart normal, but for ecchymoses in left 

 ventricle. The peritoneal fat flaccid, mottled and watery. Evidence of 

 commencing putrefaction was present on opening the abdominal cavity, 

 but elsewhere there was none. 



Case II. — Two-year-old wether, fat. — Still warm when found. No 

 evidence of struggling. In subcutaneous tissues, especially of neck, irregular 

 patches of sero-sanguineous, semi-gelatinous eflusion. Pre-scapular glands 

 enlarged, deeply haemorrhagic and oedematous. Abdomen : about a pint 

 of serous exudate, slightly blood-tinged. Peritonitis with flaky deposits on 

 posterior surface of liver, and at border, also on gastro-hepatic mesentery. 

 Liver mottled with areas of necrosis ; organs smaller than normal ; biliary 

 cirrhosis marked ; a few distoma present in gall-bladder ; hepatic glands 

 enlarged, but not haemorrhagic. Rumen, tympanitic. Abomasum ; mucosa 

 deeply congested but no ulceration or visible necrosis ; submucosa slightly 

 oedematous. Intestines empty but for small quantity of clear, glairy fluid in 

 small bowel. Kidneys congested ; urine in bladder albuminous and cloudy. 

 Spleen enlarged and diffluent. Thorax : 8-10 oz. of sero-sanguineous 



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