612 PROCEEDINGS OF SECTION G2. 



later. Post-mortem : bloody froth from nostrils, a little blood oozing from 

 mouth and vagina ; intense subcutaneous oedema of limb, oozing through 

 skin of hock ; some gas formation, but no odour of putrefaction ; skin of 

 thigh slightly discoloured but not necrosed ; muscles dark and serum in- 

 filtrated ; subcutaneous tissues show marked haemorrhages here and there. 

 Abdomen : uterus congested and oedematous ; peritoneum contained a small 

 quantity of sero-sanguineous effusion. Abomasum : submucosa slightly oede- 

 matous, large haemorrhagic patch on serous surface. Mucosa catarrhal but not 

 injected or congested ; spleen normal ; kidneys congested ; liver congested, 

 here and there on posterior surface some gas formation. Thorax : about 

 6 oz. of blood-stained effusion. Lungs congested and oedematous ; bronchi 

 and trachea congested with haemorrhages ; pericardium distended with clear 

 semi-gelatinous effusions ; petechise on epicardium and left endocardium. 

 Microscopical examination of fluids showed bacilli often in pairs, apparently 

 pure, but for a few longer organisms of same thickness ; m congested and 

 haemorrhagic muscle numbers of bacilli sporulating and no long organisms. 

 The blood showed no organisms, but after incubation in pipettes numerous 

 bacilli morphologically similar to those described were found. 



Sheep 17 (3 years old merino, in good condition). — Inoculated with 0'25 

 CO. sub-culture from muscle of sheep 12. As controls, guineapig 113 was 

 inoculated with 1 cc, guineapig 120 with O^S cc, and rabbit 34 with 0-5 cc. 

 of the same culture, all of which died within 20 hours. 16 hours after inocula- 

 tion sheep 17 was found not eating, the leg lame, and slightly swollen 

 and micturating freely. Temperature 101.5. The animal remained in this 

 condition throughout the day and was found dead 38 hours after inoculation, 

 death having apparently occurred some hours previously. Post-mortem : 

 inoculated region swollen, puffy, and livid ; subcutaneous oedema extended 

 forward to the xyphoid, sanguineous at the point of inoculation, but clear 

 elsewhere ; muscles of limb dark in patches, elsewhere pale and friable; 

 no definite odour. Peritoneum : small quantity of cloudy sero-sanguineous 

 effusion ; spleen pulpy, dark and somewhat enlarged ; liver mottled, 

 especially near gall-bladder with areas of necrosis, varying in size from 1 

 to 2 cm. Abomasum : mucosa deeply congested, with surface covered by a 

 thin diphtheritic membrane ; here and there small areas of superficial necrosis 

 and patches of haemorrhage. Kidneys normal. A few ounces of pleural 

 effusion ; lungs oedematous, with many sub-pleural petechias. A little 

 pericardial clear effusion ; many epicardial petechias. Microscopicalexamina- 

 tion : many bacilli at region of inoculation and in muscle ; a number in 

 necrosed liver areas, none visible in smears from elsewhere. 



Sheep 18 (4 year old crossbred ewe). — Inoculated with 0-5 cc. culture 

 from liver of guineapig 102. In four hours the animal showed uneasiness. 

 Temperature 103.5. The following morning, 19 hours after inoculation, 

 found dead. Post-mortem : inoculated limb showed much oedematous swelling, 

 with some gas, extending from coronet up limb, and forward to sternum, 

 no skin discolouration. On incising skin, very slight odour, which dis- 

 appeared almost immediately. Muscles variegated with greyish and dark 

 areas. Uterus congested, swollen and oedematous. About 6 oz. of peri- 

 toneal fluid, but slightly blood-stained ; spleen enlarged and pulpy; liver 

 pale and shrunken ; stomach normal ; pleura and pericardium contained a 

 small quantity of clear effusion. Heart showed large blood extravasations 

 under epicardium, especially of auricles and appendages ; and many petechiae 

 in left endocardium. Microscopical examination of fluids showed typical 

 bacilli in subcutaneous oedema and muscles ; similar bacilli, but also 

 long undulating filaments in peritoneal fluid. The pleura and blood also 

 contained typical bacilli. 



GUINEAPIGS. 



Guineapigs 64 and 65 were inoculated with a small quantity of blood 

 from Case I. and guineapigs 66 and 67 with blood from Case II. Guineapig 

 65 succumbed in 40 hours, examination showing a large oedematous swelling 

 at the region of inoculation and extending forward to the shoulder. Few 



