" BRAXY " SHEEP DISEASE IN AUSTRALIA. 613 



organisms were present in the oedema, these being chiefly short bacilli. The 

 other guineapigs developed only slight and passing swelling of the inoculated 

 limb. 



Giiineapig 69. — Inoculated with 0'05 cc. spleen pulp from sheep 6. On 

 incubation at 37° this spleen pulp developed a putrefactive odour, and besides 

 the double bacilli which sporulated, large double sporulating bacilli also de- 

 veloped. This animal developed slowly a swelling at the seat of inoculation and 

 died in five days. Post-mortem examination : a mass of necrosed material at 

 site of inoculation ; oedematous effusion extending forward to thorax in 

 subcutaneous tissue ; small quantity of semi-gelatinous effusion in peri- 

 toneum ; intestines congested, and small bowel oedematous ; spleen enlarged ; 

 liver pale ; otherwise normal. Smears showed mixed bacilli, with some 

 polymorphs in the necrosed tissue. The oedema contained chiefly the double 

 bacillus of braxy type. Similar bacilli were present in the liver, spleen, etc., 

 but the blood was apparently sterile. 



Guineapig 73. — Inoculated with 0'25 cc. anaerobic culture in glucose serum 

 broth from uterus of sheep 7 showing deposit with little cloudiness and little 

 gas formation. Bacilli apparently of braxy type morphologically, but a 

 large number do not stain by method of Gram. Animal found dead 20 

 hours later. Post-mortem examination : sero-sanguineous fluid in sub- 

 cutaneous tissues of inoculated limb, and a little peritoneal fluid ; a putrid 

 odour present. Smears show mixed bacilli — some Gram positive, others 

 not ; a number of long bacilli also present. 



Guineapig 74. — Inoculated with 0"05 cc. peritoneal fluid from sheep 7, 

 showing numbers of typical braxy-like bacilli, many sporulating. Found 

 dead 18 hours later ; iDody cold. Post-mortem examination : sero-sangui- 

 neous effusion into subcutaneous tissues of inoculated limb ; slight clear peri- 

 toneal effusion ; otherwise normal. Examination of subcutaneous effusion 

 showed many typical bacilli, which were also present in peritoneal fluid and 

 spleen, but along with these were many long filaments. 



Guineapig 75. — Inoculated with 0-05 cc. pericardial fluid from sheep 6' 

 (as used for sheep 9). Animal died 20 hours after inoculation. Post-mortem 

 examination : much subcutaneous oedema extending along inferior portion 

 of body to point of thorax ; no gas and no odour ; muscles slightly pale at 

 region of inoculation ; liver mottled with necrosed areas ; spleen enlarged 

 and dark ; little peritoneal fluid ; sero-sanguineous pleural effusion ; clear 

 effusion in pericardium. Many typical braxy-like bacilli were found in fluid 

 of subcutaneous tissues and a few in spleen. The liver necrosed areas con- 

 tained besides these many long filaments. The heart blood was apparently 

 sterile. 



Guineapig 76. — Inoculated with OT cc. heart blood of sheep 7, which 

 after incubation showed many sporulating bacilli with putrefactive odaur. 

 Animal found dead 18 hours afterwards. Post-mortem examination : 

 gangrene of skin and tissues at region of inoculation ; little sero-sanguineous 

 subcutaneous effusion ; no effusions in serous membranes ; intestines deeply 

 congested ; liver pale. Smears from subcutaneous fluid, peritoneum, spleen, 

 and liver showed bacilli irregular in length with many long filaments, the 

 general appearance being that of malignant oedema organisms. (Compare 

 guineapig 75.) 



Guineapig 11. — Inoculated with 0*2 cc. subcutaneous oedema of sheep 9 

 (as used for sheep 12). 



Guineapig 78. — Inoculated at same time with OT cc. blood from sheep 9, 

 after incubation, and showing bacilli of braxy type, some sporulating ; also 

 a few longer bacilli. 



Animals found dead in 20 hours. 



Post-mortem examination on these animals showed a similar picture : — 

 infiltration of subcutaneous tissues of limb and abdomen, with clear oedema; 

 no discolouration, no odour and no gas ; small quantity of somewhat cloudy 

 peritoneal fluid ; spleen dark, but not enlarged ; small intestines distended 

 with oedematous effusion into lumen. Liver of 78 showed patch of greyish-yellow 



