614 PROCEEDINGS OF SECTION G2. 



necrosis. Smears showed typical braxy-like bacilli in subcutaneous effusions. 

 In peritoneal effusion, similar bacilli, but also long filamentous organisms. 



Guineapig 81. — Inoculated with 0'25 cc. second subculture in serum 

 broth under oil from blood of guineapig 77, which, after incubation, showed 

 typical bacilli. Animal dead in 20 hours. Post-mortem examination : sub- 

 cutaneous oedema, peritoneal fluid, intestinal effusion, etc., as in guinea- 

 pig 77. 



Guineapig 98. Inoculated with O'l cc. third subculture in serum broth 

 under oil from blood of sheep 12 after incubation. Animal found dead in 

 20 hours. Post-mortem examination typical with patches of necrosis in 

 liver, effusions, etc. 



Guineapig 102. — Inoculated with 0*75 cc. blood of sheep 12 after incu- 

 bation (as control of guineapig 98). Animal found dead 20 hours later. 

 Post-mortem examination : as in guineapig 98, but no liver necrosis. 



Guineapig 107. — Inoculated subcutaneously with 0-25 cc. subculture 

 from blood pipette of sheep 18. Death in less than 20 hours. Usual post- 

 mortem picture. 



Guineapigs 113 and 120. — Inoculated with O'l cc. and 0'3 cc. respectively 

 with second broth subculture of muscle of sheep 12, being control to sheep 17 

 and rabbit 34. Result, death in 16 hours. Post-mortem examination : 

 much oedema and some gas formation, with haemorrhage in muscles ; small 

 intestines oedematous ; spleen enlarged and congested ; liver congested. 



III. — Experiments on Other Animals. 



Calf. — A calf six months old, in good condition, was inoculated behind 

 the shoulder with O'Scc. 24 hours' old first culture from sheep 12. The 

 following day a very slight swelling, with no lameness, was visible ; tem- 

 perature, 102°. This swelling rapidly disappeared, the animal continuing 

 normal. (^Compare calf 5, Tasmania.) 



Rabbits. — Rabbit 26, inoculated with OT cc. oedema from leg of guinea- 

 pig 102 (which succumbed in 20 hours with OT cc. blood of sheep 12 after in- 

 cubation) ; remained normal. 



Rabbit 29, inoculated with 0"25 cc. same culture as used for guineapig 

 107 ; remained normal. 



Rabbit 34, inoculated with O'Scc. second broth culture as used for 

 guineapigs 113 and 120; dead in 16 hours. Post-mortem examination: 

 much subcutaneous oedema, with little gas and no definite odour, extending 

 forward to the shoulder, clear and semi-gelatinous ; a small quantity of 

 peritoneal effusion ; spleen enlarged and dark, otherwise normal. 



Rabbit 40, inoculated with 0-2 cc. first culture (muscle), remained 

 normal. Guineapig 171 inoculated with 1 cc. same culture dead in less 

 than 20 hours. 



Pigeons. — Two pigeons inoculated with 0'05 and OT cc. respectively 

 were found dead 18 hours later. Post-mortem examination: beyond an 

 oedematous infiltration, with haemorrhage of the muscles at the seat of 

 inoculation, no pathological change. 



Fowls. — Two fowls inoculated with similar doses remained normal. 



IV. — Cultures in Glucose Serum Broth — Rapid Loss of Virulence — 

 Immunity Produced. 



Guineapigs 130 and 131. — Inoculated with OT cc. first subculture in glucose 

 serum broth, 24 hours old, from sheep 12 ; both found dead in 20 hours. Post- 

 mortem examination : typical picture, with much subcutaneous oedema, little 

 gas, a little effusion into small intestines and in peritoneal cavity, one showing 

 a few necrotic patches of liver, the other none. Seven days later this 

 culture was again employed in a similar dose on Guineapig 135. In this case, 

 however, no change was observed till the third day, when a slight swelling 

 was noticed, but the animal was otherwise normal. Death occurred six 

 hours later, when post-mortem examination showed the usual picture. 



