" BRAXY " SHEEP DISEASE IN AUSTRALIA. 615 



Sheep 20 and 25 (2 years old). — Sheep 20 inoculated with 0*25 cc. fourth 

 subculture from muscle of sheep 12. No swelling or other abnormality was 

 observed the following day. The sheep was then inoculated with 0.25 cc. 

 first subculture, seven days old, from sheep 12 (as employed originally for 

 guineapigs 130 and 131). (See also guineapig 135.) Four days later, the 

 sheep having remained normal, it was again inoculated with 3 cc. fourth 

 subculture, eight days old ; the sheep remained normal. Fifteen days later 

 re-inoculated with 0'25cc. two-days' old second subculture, sheep 25, of 

 same age and breed, receiving a similar dose, and guineapig 144 receiving O'l cc. 

 as controls. Sheep 20 remained normal. Sheep 25, the following morning, 17 

 hours after inoculation, was very lame, with considerable subcutaneous 

 cedema of the inoculated limb ; temp., 106.8° (temp, of sheep 20, 103.7°). 

 The guineapig was prostrate and died about 26 hours after inoculation, the 

 post-mortem examination showing a typical condition. Sheep 25 gradually 

 developed graver symptoms and died about 50 hours after inoculation. 

 Post-mortem examination showed the usual condition ; much oedema 

 of inoculated limb, which implicated the subcutaneous tissues of abdomen 

 forward to the xyphoid ; intestines injected, especially small intestines, which 

 contained some extravasated serous fluid ; abomasum, submucosa oedema- 

 tous, mucosa deeply congested with patches of surface necrosis ; little 

 effusion in peritoneum and pleura ; liver small, very pale and waxy ; spleen 

 not enlarged, but dark ; lungs oedematous, ecchymoses under pleura, espe- 

 cially posterior portion ; pericardium distended, with deeply blood-tinged 

 effusion ; petechiae of epicardium and of left endocardium. 



Sheep 31 (2 years old). — Inoculated with 3 cc. eight days old third sub- 

 culture from sheep 12 in glucose serum broth, and guineapig 149 inoculated 

 with 1 cc. of same culture. Result: sheep developed no lameness or swelling, 

 and the temperature did not rise above 103°. The guineapig developed a 

 slight swelling, from which it rapidly recovered. Twenty days later sheep 31 

 re-inoculated with 0-25 cc. first culture 24 hours old from muscle of sheep 12 

 in ordinary serum broth. Guineapig 149 was re-inoculated with 0-15 cc. of 

 Same culture, and guineapig 161 was inoculated with 0T5 cc as control. 

 Both'guineapigs died in less than 20 hours. Post-mortem examination typical. 

 Sheep 31 the following day showed no swelling and but slight lameness. The 

 temperature was 104.5, which next day dropped to 102.8. (Further inocula- 

 tions are recorded in text.) 



Sheep 30 (3 years old). — Inoculated with 5 cc. seven days' old first sub- 

 culture (which when one day old had proved fatal for guineapig 151 with 

 a dose of 0.1 cc). Guineapig 153 inoculated with 1 cc. of same culture. 

 Guineapig 153 was found dead in 18 hours ; typical post-mortem. Sheep 30, 

 following day, showed considerable swelling of region of inoculation, extending 

 down the limb, accompanied by definite lameness. Temperature 104*2. 

 Animal, however, had fair appetite. The following day the lameness and 

 swelling had disappeared. Temperature 103 "2. Fifteen days later sheep 30 

 xe-inoculated with 0'25 cc. 24 hours' old first culture in serum broth from 

 muscle. The sheep the following day was slightly lame, but there was little 

 swelling. Temperature 104-6. The lameness increased somewhat, but 

 appetite remained. Next day the normal was resumed. Temperature 102'5. 

 For further inoculations, including cross experiments, see text.) 



V. — Filtered Cultures. 



A five days' old culture containing many bacilli (some degenerating) from 

 -muscle of sheep 18, and which proved fatal for guineapig 111 in 20 hours, 

 was filtered through aChamberland B filter. Of the filtrate 5 cc. was injected 

 intraperitoneally into guineapig 113. The animal remained normal. Twenty- 

 four days later it received subcutaneously O'l cc. first broth culture from 

 muscle of sheep 18, as used for guineapig 120 and rabbit 34. Death occurred 

 in 16 hours. Post-mortem examination typical. 



