Microscopic Examination of Blood. 319 



JViicIei. — Time would not permit a thorough examination 

 by the use of stains, and in order to gain as much information 

 as possible in the time which could be devoted to this part of 

 the investigation, a weak solution of acetic acid was emplo3'ed. 

 This fact must be held in mind in making the application of 

 my observations on these nuclei. 



The three largest leucocytes seen, those mentioned before 

 as being r^Vuof an inch, or about i8,«, in diameter, were mono- 

 nuclear, the nuclei being of the first variety. The nuclei 

 were ssV^of an inch, or about io«, in diameter. Thus it will 

 be seen that the nuclei of these three immense cells were as 

 large as the average leucocyte. 



The leucocytes containing nuclei of t'ne second variety were 

 of large size, all exceeding suVit of an inch, or about 1 3,^. 



The leucocytes containing nuclei of the third and fourth 

 varieties were of medium size, and ranged from jsVa of an 

 inch, about lO//, to sis'inrof an inch, or about i3,«. 



The lymphocytes were much smaller, the largest not ex- 

 ceeding 2sW of an inch, about lOu. Some were but ssVt of an 

 inch, about S;^; in other words about the size of the nuclei 

 of the mononuclear and transitional leucocytes. 



As to the relative dumber of the various varieties, no 

 attempt was made to estimate the percentages. Occasionally 

 every cell in a field was examined, and almost every one 

 would be of the large mononuclear type. Again, other fields 

 contained almost an equal number of the larger mononuclear 

 and the transitional leucocytes. The Ij^mphocytes were by 

 no means very numerous. It is to be regretted that oppor- 

 tunity was not afforded to make a thorough investigation of 

 the nuclei of this blood, and estimate the per cent, of the 

 various varieties of leucocytes, but the patient left the hospi- 

 tal March 8th, and the writer was not permitted to complete 

 his studies by the use of stains and various reagents. 



Amoeboid Movement. — The blood was examined on the 

 warm stage, March 6th. The temperature was maintained 

 at about 350 C. The movement was quite sluggish as com- 

 pared to that of leucocytes in normal blood. 



