79 
was that with the exception of the Helminthosporium, these fungi were 
very rarely present, and then only a single colony or part of a mixed colony 
on occasional plates. Alternaria occurred with remarkable rarity; only 
two or three colonies among several thousand. Fusarium was found in 
only a few colonies and so mixed that it was isolated with difficulty. Ep- 
icoccum occurred in two colonies; Phyllosticta also in two colonies (two 
species). 
A Helminthosporium, however, appeared in every plate and from 
nearly every bit of tissue used, no matter how great the care in securing the 
inoculum. On many plates this Helminthosporium (which throughout 
this article I designate as H. No. 1) appeared in pure culture Thus it 
may be said that the Helminthosporium was universally present in the 
plates; that it was the only organism that was present with any constancy; 
and that all other fungi were obviously strays.* Though conidia were 
never found in great numbers on plants brought in direct from infested 
fields, when the plants were placed in moist chamber for two or three 
days conidia developed in abundance. This was also the case with portions 
of wheat stems which had been placed in bichloride of mercury for ten 
minutes and then placed in moist chamber for several days. In passing 
it may be remarked that although great numbers of nematodes and amebae 
appeared in the plates there is no reason to believe that they had any relation 
to the disease under discussion or to any diseased condition. 
GROWTH OF THE CausAL FunGuUS ON Various MeEptIa 
Since the characters exhibited by various Helminthosporiums when 
growing in artificial culture have been considered as of importance as a 
means of distinguishing one species, variety, race, or strain from another, 
many media were employed in the present study. This was done in part for 
the purpose of comparing the growth characters of the Helminthosporium 
with characters reported by others in connection with other forms; in part 
with the hope that some of the media tested might give emphasis to cer- 
tain characters and thus serve to differentiate between species or strains 
of the forms under observation. 
The following notes are, in the main, statements of the characters 
presented by the foot-rot Helminthosporium (H. No. 1), though for the 
purpose of comparison notes are added regarding the growth of several 
*A letter from Professor Hoffer written in May, 1919, tells me of a similar result from platings of wheat 
foot-rot from Indiana, and similar reports reach me from several other sources. 
