J 14 Bl'LLETIN DK TlllC lU KliAT Ob' KISHEKIES. 



l-tG. lb. A spoa)Cyst of M. musciili fa>m ;i sn\ciir of liisciiscd intogunKiU of Uio moulh ;iik1 head 

 in front of the eyes. Elsewhere the siwrocysts hove less cytoplasm. It is the only one encountered 

 in this condition. The faiUire of the nuclei to take the stain is chixrncteristie. The myxospore is 

 iiuni.iUire, being less slender than older niyxospores. The details of the polar capsules arc verj' trims- 

 parent and stain dark bhie. while the spore wall is a ver>- pale blue. The vacuole and sporoplasin are 

 painiiueiil. but tlie nuclei of the spore can not be clearly discerned. SjTOrocyst, 17.811 by .J^i.S/i; siwre. 

 I4.S« by 7.4/t; jwhu- capsule, 7.4/1 by 3.J11. There iu-e iji-i4 spirals in the lilanient. Fixation: Absolute 

 alcohol, ether, corrvwive sublimate, acetic acid. Stain: Mayer's lutin.Uein. methylene blue, orange G., 

 eosin. (Xaooo.) 



Flo. a;. Asporobhist of .1/. miiscuU from a fresh smcitf of degenerated nuisele taken from a deep 

 cimty (tlie same iis fig. jo). H;>sily distinguished from tissue cells by tlie three nuclei. Protoplasm 

 contiiins nuich coarsely gnuiular matter. ^Dra\\^l free-luuid, not to scale.) 



1^0. jS. Mvxospore from the s;ime slide as figure 26. The mature spore, when comp.treil with that 

 in the pansix'-RibUist , is longer and more pointed at the polar end. The \acuole is pmbably ;ui iodi- 

 nophilous structure. The coiled filaments make 11 to i.- tunis. The polar cajisule wall is visible, but 

 tlie siHirc wall c;m not be clearly seen. The valves and sutures are als<i indistinguishable. While there 

 are as numy as u blue iuul green bodies present, one can not be sure that all of them ;u-e nuclei. Seven 

 oreight bodies are moderately conspicuous. Two lie in the w.iU of the pol.ir capsules .uid are doubtless 

 tl>e capsule nuclei. 14.811 by h.a.-t. (Xaooo.) 



1^0 . JO . A myxospore of M. w usi-u li from large sores on each side of tlie tail of a Fii nJu lus heleroclil us, 

 caud;U fin cntirclv gone. Fixed in absolute alcohol luid ether, stained with methylene blue. Six 

 imstained nuclei in the sporoplasn\ and one large vacuole. Filament discharged. Spore, 7.4/1 by 16.4/1. 

 Pohir capsule, .v.'/i by 7.411. (.\jooo.l 



Fig. ;o. Diagram of the cross section of a fresh myxosjx>re of .1/. musculi as if seen from Uie end. 

 The specimen was Iving sii as to present the edge of the valves to view. It is obviously flattened. The 

 polar capsules also appeared to be, but one c;ui not be certain alxnit this. The sutures are str.iight ;md 

 symmetrical. Fixation: Alcohol, ether, formalin; Giemsii stain. t^This drawing not made to scale.') 



[Figures 31 to ,^4 are all from the s;ime smear preparation of disciised muscle from a dead fish, being 

 one of those taken fa>m jsir no. i (see pp. 105, 106),] 



I'lo. ^1. Pansjioroblast of Cliloiomyxuiii Juinliili embedded in a degenerated muscle fiber. The 

 contained myxosixire h,»s taken up tlie stain, but the protoplasm of the p;msporoblast is absolutely 

 dcN-oid of visible structure. Note the even contour of the characteristic lobose pseudopodia. 15.2/1 by 

 13/1. (Xaooo.) 



Fig. 33. One of a group consisting of free young luyxiispores of C fuiiduli. Like the mature 

 mjTcospores, tliey stain readily, but tlieir nuclei ;u-e not ditTercntiated. They iut;, as a rule, not quite 

 so irregular, but tlie pseudopodia are al\va>-s small imd angular. Note the contrast between these ;md 

 the pansporoblasts. 3.7/1 by 4.5.11. (X^ooo.) 



FiG. 33. JIvxospore of C. funduli. The outline is approximately circular. The sporoplasm is 

 homogenixius bvit dense ;iroimd the four [wUir capsules, doubtless because of tlie greater thickness at 

 tliis jv>int. The four nuclei iue always associated with the pohu capsules, hence are doubtless capsule 

 nuclei. Diameter. S.o/i. (Xjooo.) 



Fig. 34. Mvxospore of C funduli seen from the side. Note the sjxiroplasm is not much denser 

 about tlie poUv capsules. The sporoplasin tapers to a blunt apex. In many it is more pointed. The 

 polar capsules have long, curved, tapering necks with the hirge ends far ap;ul. The capsule nuclei 

 alone stain. 8.3/1 by 6.7/1. (Xiooo.) 



Fig. 35. A fresli spon:>bl;ist of .^/. musculi from the Siime slide as figures 30 and 37. The cyto- 

 pUism is rich in gr.mules. The nucleus is ver>- hu^e imd has a conspicuous kar>-osome. (Not drawn 

 to scale.) 



Fig. 36. An isolated epidermal cell derived from a mass ne;ir the ni.irgin of an ad\Tmced uleer, 

 most of which have numerous unst.iined bodies like tlK%se in the muscle fibers (fig. 13, pi. xxl. From 

 sections. The epidermal cell is not typical in appe;ir.mce, but the unsliiined bodies are, imd are iden- 

 tical to tliose in the adjacent slightly atrophied epidermis. (Xaooo.) 



