MOVING PHOTOMICROGRAPHY— KAZEEFF 



327 



The improved moist chamber (of Ranvier) and the oil chamber. The 

 improved moist chamber (fig. 1) consists of a glass slide on which is 

 attached with sealing wax a flat glass ring about 15 millimeters in 

 interior diameter and 1 millimeter thick. This ring has an opening 

 2 to 3 millimeters wide. It is covered with a cover glass that is 

 sealed to the crown by means of a thin layer of vaseline. The medium 

 and the objects to be observed are introduced through the lateral 



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FiGUBK 1.— Moist chamber. 



opening of the ring by means of a fine pipette. When the pipette 

 has been removed, the lateral opening is closed up with a stopper of 

 vasehne. 



The provision of enclosed air in this moist chamber is generally 

 suflScient even to satisfy the need of larvae during their hatching. If 

 necessary, the air and the medium can be renewed through the same 

 lateral opening. 



FiGUEE 2.— Oil cliamber. 



The oil chamber (fig. 2) consists of a large drop of oil of vasehne 

 placed on a cover sUp held horizontally by means of a Httle support 

 placed under the microscope (figs. 2-A and D). The center of the 

 drop of oU just to the point of contact with the glass is pierced by the 

 point of a fine pipette containing the medium and the living organisms. 

 The pipette is connected to the mouth of the operator with rubber 

 tubing. By hghtly blowing into the tubing, the aqueous Hquid is 

 made to leave the pipette and spread out on the glass under the oU 



