478 Miscellaneous. 



In the Orcliestice the inoculations do not succeed so easily because 

 the operation is more delicate ; but the animal longer retains its 

 muscular power : an Orchestia inoculated on the 12th still jumped 

 on the 19th, although it was in full phosphorescence. The Talitri 

 and Orchestia' in which the inoculation does not succeed remain in 

 perfect health long after their congeners are dead ; the puncture, 

 when well made, therefore is not serious in itself. 



I have inoculated examples of Hyale NUssoni, Rathke, with perfect 

 success ; in these little Amphipoda phosphorescence is produced in 

 forty-eight hours. Specimens of Ligia ocecmica, Linn., though more 

 resistant, also gave a favourable result. Of six Ligice unsuccessfully 

 inoculated on the 10th and reinoculated on the 16th only one was 

 infected ; but after the 20th it presented an admirable spectacle. 



I have also succeeded in inoculating crabs (Carcinas mcenas, 

 Linn., and Platyonyclms latijjes, Penn.). In these animals, how- 

 ever, the morbid phenomena are much more complex, and I will 

 notice them in a subsequent communication. At the same time I 

 will describe my experiments in the culture of the Bacteria in arti- 

 ficial media. — Com/ptes Hendus, September 23, 1889, p. 503. 



On the Parasitic Castration of the Typhlocybae hy a ffymenopterous 



Larva (Aphelopus melaleucus, Dalm.) and by a Dipterous Larva 



(Atelenevra spuria, Meig.). By M. A. Giaed. 



The Hymenopterous and Dipterous larv;© parasitic upon Typhlocyha 

 noticed by the author in a former communication * belong, the 

 former to Aphelopus melaleucas, Dalm., the latter to Atelenevra 

 spuria, Meig. (A. velutina, Macq., Chalarus spurius, Schin.). 



These insects, like their hosts the Typhlocybce, have two genera- 

 tionsin the year: one, proceeding from pupse formed during the second 

 fortnight in June, comes out at the beginning of July ; the other 

 infests the second generation of Typhlocyha, enters the pupa state 

 towards the end of September or in October, and probably passes 

 the winter in that state, producing the perfect insect in the following 

 spring. 



Combining these observations with those of Ferris (on the parasi- 

 tism of Drifinus pedestris, Dalm., upon Athysanusmaritimus, Ferris) 

 and of J. Mik (on the parasitism of Oonatop>us pilosas, Thorns., upon 

 Deltocephalus xantlionearus, Ficb.) it seems probable that the Froc- 

 totrupians of the family Dryiuidae are generally parasitic upon 

 Homoptera of the family Jassidae. 



On the other hand, as regards the Diptera, the present observation, 

 especially in conjunction with Boheman's statements, particularly 

 as to the infestation of Cicadula virescens. Fall. (Thamnotettix sul- 

 phurella, Zett.), by the larva of Fijyiinctdiis fascipes. Fall., makes it 

 probable that the Diptera of the family Fipunculidoe are also generally 

 parasitic upon Jassidoe. 



The Typhlocyhoi with yellow or whitish elytra form a small group 

 of species often living side by side upon the same trees, and resem- 

 bling each other so closely that it is almost impossible to distin- 

 guish them. Mr. James Edwards, of Norwich, has recently called 

 attention to the very distinct differential characters presented by 

 the male genital armature in these different species. In accordance 



* Comptes lieudus, July 8, 1889, p. 79 ; see Annals, mprit, p. 254. 



