decided to submit several colonies for detailed examination. 

 This was very kindly carried out by Mr. Smith, of the Bacterio- 

 logical Department in the School of Pathology, whose report 

 is embodied in this one. He examined a plate of each sample, 

 on which white colonies were numerous. These were regarded 

 as significant of recent pollution, and were subcviltured Avith 

 the following results : — Lactose A, Saccherose +, Glucose +, 

 Mannite +, Maltose +, Indol — , Motihty +; A = Acid, + = 

 acid and gas, — = no reaction. The organisms were identified 

 as Friedlander's Pneumobacillus. No organisms of knov.n 

 pathogenicity A\ere found. The relative proportions of 

 occurrence of the different organisms studied were Pneumo- 

 bacillus Friedlander -f, B. Coli -| — | — \-, Streptococci -j — \-. 



The most significant of these results is the relative abun- 

 dance of Streptococci. This organism exists in large numbers 

 in human faeces, and its association with other faecal bacilli 

 not only indicates serious, but also recent, pollution as it 

 is a delicate organism and only survives for a short time when 

 isolated. 



North Training Wdll. lol miles frmn, Preston. Docls. 

 No. 2 Gas Buoy. 



I. — 24 hrs. A\hite, 100 small red, large red colonies. 



Department and not counted, but 

 contained a large number of small red 

 colonies. 

 VI. — 24 ,, 1 ,, 177 small red, 2 large red colonies. 



After 48 hours a great many more microscopic red colonies 

 became visible, in addition to those alreadv counted, so that 



