these counts only represent the minimum number of organisms 

 present. The small colonies which were counted Avere those 

 easily visible to the unaided eye, and were mostly about ^th" 

 in diameter or less, and Mere well scattered. 



Result of Incubation in Bile Salt Lactose Litmus Broth : — 



Four tubes were inoculated as before, with the following 

 results : — 



Tube I., after 24 hours' incubation, showed an 

 acid reaction but no gas formation, but after 48 hours 

 was gassing freely. There was no reaction in Tubes 

 II., III. and IV. after 48 hours. 



These four tubes Avere incubated for a long period, 

 but no further reactions Avere observed. 



The result of the examination of the suspected Avhite 

 colonies on Plate V. by Mr. Smith Avas as folloAvs : — Lactose 

 A, IMannite A, Maltose A, Indol +, Motility +. A= acid 

 production. An agglutination test against Bacillus typhosus 

 and Bacillus dyscnterice Avas carried out with negatiA'e results. 

 The organism examined Avas identified as Bacillus pyogenes 

 foe-tidus. Other bacilh present Avere Bacillus coli and Strepto- 

 coccus. No organism of knoAvn pathogenicity Avas found. 

 Here again is CAidence of faecal pollution, but of a different 

 character from the Church Scar sample. The main feature 

 is the relative abundance of lactose-fermenters and the absence 

 of non-lactose fermenters. 



Comparison of the two Samples. 



In comparing the tAvo samples, the following explanations 

 should be carefully noted. The average number of organisms 

 per mussel in the Church Scar sample is estimated from four 

 plates after 48 hours' incubation. The fifth plate was dis- 

 carded OAA'ing to a general fusion of the colonies in the centre, 



