﻿LABORATORY 
  OBSERVATIONS 
  AND 
  EXPERIMENTS. 
  25 
  

  

  May 
  27; 
  June 
  6; 
  June 
  15. 
  

  

  May 
  27; 
  June 
  6; 
  June 
  15; 
  June 
  21. 
  

  

  Thirteen 
  jars, 
  started 
  June 
  2, 
  each 
  jar 
  being 
  infected 
  with 
  four 
  fungus- 
  

   covered 
  bugs 
  from 
  the 
  preceding 
  jars, 
  are 
  recorded 
  as 
  follows: 
  

  

  June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

   June 
  2 
  

  

  June 
  11; 
  June 
  20. 
  

  

  June 
  11; 
  June 
  19. 
  

  

  June 
  11; 
  June 
  19. 
  

  

  June 
  7; 
  June 
  15. 
  

  

  June 
  11; 
  June 
  15; 
  June 
  20. 
  

  

  June 
  11; 
  June 
  19. 
  

  

  June 
  11; 
  June 
  19. 
  

  

  June 
  11; 
  June 
  20. 
  

  

  June 
  15. 
  

  

  June 
  11; 
  June 
  19. 
  

  

  June 
  11; 
  June 
  19. 
  

  

  June 
  11; 
  June 
  15; 
  June 
  20. 
  

  

  June 
  11; 
  June 
  15; 
  June 
  20. 
  

  

  One 
  jar, 
  started 
  June 
  4, 
  ran: 
  

   June 
  4; 
  June 
  11; 
  June 
  20. 
  

  

  One 
  jar, 
  started 
  June 
  5, 
  ran: 
  

   June 
  5; 
  June 
  15; 
  June 
  20. 
  

  

  Four 
  jars, 
  started 
  June 
  6, 
  are 
  recorded: 
  

   June 
  6; 
  June 
  15; 
  June 
  20. 
  

   June 
  f>; 
  June 
  15; 
  June 
  19. 
  

   June 
  6; 
  June 
  15. 
  

   June 
  6; 
  June 
  19. 
  

  

  The 
  bugs 
  put 
  into 
  the 
  jars 
  on 
  the 
  several 
  dates 
  were 
  from 
  all 
  parts 
  of 
  the 
  

   chinch-bug 
  district 
  of 
  the 
  State. 
  They 
  were 
  for 
  the 
  most 
  part 
  just 
  received 
  

   from 
  the 
  mail 
  and 
  were 
  in 
  vigorous 
  condition. 
  They 
  were 
  kept 
  supplied 
  with 
  

   green 
  wheat. 
  While 
  the 
  bugs 
  in 
  the 
  infected 
  jars 
  were 
  dying 
  at 
  intervals 
  of 
  

   5 
  to 
  10 
  days, 
  bugs 
  in 
  isolated 
  check 
  jars 
  remained 
  alive 
  and 
  vigorous. 
  

  

  By 
  June 
  20 
  the 
  demand 
  for 
  infected 
  bugs 
  was 
  so 
  large 
  that 
  the 
  jar 
  method 
  

   of 
  infection 
  required 
  more 
  attention 
  than 
  we 
  were 
  able 
  to 
  give 
  it. 
  The 
  results 
  

   of 
  our 
  separate 
  lots 
  were 
  so 
  uniform, 
  and 
  the 
  Sporotrichum 
  so 
  vigorous 
  and 
  

   ever 
  present, 
  that 
  the 
  further 
  watching 
  of 
  separate 
  lots 
  seemed 
  useless. 
  Ac- 
  

   cordingly, 
  June 
  20, 
  a 
  large 
  glass 
  case 
  was 
  arranged 
  with 
  damp 
  sand 
  three 
  

   inches 
  deep 
  over 
  the 
  bottom. 
  About 
  10 
  dead 
  bugs 
  covered 
  with 
  Sporotrichum 
  

   were 
  scattered 
  over 
  the 
  sand, 
  and 
  large 
  quantities 
  of 
  live 
  bugs 
  from 
  the 
  field 
  

   were 
  put 
  in, 
  with 
  plenty 
  of 
  green 
  wheat. 
  In 
  nine 
  days 
  the 
  bottom 
  of 
  the 
  

   case 
  was 
  thickly 
  sprinkled 
  with 
  white-fungus-covered 
  bugs, 
  and 
  in 
  13 
  days 
  

   only 
  a 
  few 
  live 
  bugs 
  remained 
  and 
  the 
  case 
  was 
  restocked. 
  The 
  infection 
  

   continued 
  to 
  work 
  so 
  rapidly 
  in 
  this 
  case 
  that 
  we 
  found 
  no 
  trouble 
  in 
  filling 
  

   from 
  it 
  25 
  to 
  100 
  orders 
  daily. 
  Vast 
  numbers 
  of 
  young 
  red 
  chinch-bugs 
  

   were 
  put 
  into 
  the 
  case 
  together 
  with 
  the 
  adults, 
  and 
  they, 
  too, 
  were 
  an 
  easy 
  

   prey 
  to 
  the 
  disease. 
  Minute 
  points 
  of 
  white 
  made 
  their 
  bodies 
  conspicuous 
  

   among 
  the 
  larger 
  flecks 
  of 
  white 
  where 
  the 
  adult 
  bugs 
  lay 
  covered 
  with 
  Sporo- 
  

  

  