﻿APPENDIX 
  "B. 
  

  

  DIRECTIONS 
  FOR 
  OBTAINING 
  AND 
  APPLYING 
  THE 
  CHINCH-BIO 
  

  

  INFECTION. 
  

  

  In 
  order 
  to 
  keep 
  up 
  our 
  supply 
  of 
  infected 
  bugs, 
  we 
  must 
  require 
  that 
  each 
  

   person 
  sending 
  for 
  infection 
  send 
  us 
  live 
  bugs 
  from 
  the 
  field. 
  These 
  should 
  

   be 
  put 
  into 
  a 
  tin 
  box 
  (a 
  baking 
  powder 
  box 
  is 
  excellent 
  for 
  this 
  purpose), 
  

   without 
  soil, 
  and 
  with 
  a 
  supply 
  of 
  green 
  wheat 
  or 
  corn. 
  The 
  box 
  should 
  

   have 
  a 
  tight-fitting 
  cover, 
  and 
  no 
  holes 
  need 
  to 
  be 
  made. 
  We 
  have 
  found 
  

   that 
  the 
  bugs 
  reach 
  us 
  in 
  the 
  best 
  condition 
  when 
  sent 
  us 
  in 
  this 
  way. 
  

  

  Upon 
  receipt 
  of 
  infected 
  bugs 
  from 
  the 
  Station, 
  a 
  shallow 
  box 
  about 
  24x36 
  

   x6 
  inches, 
  with 
  tight 
  joints, 
  should 
  be 
  provided. 
  Sprinkle 
  the 
  whole 
  inside 
  of 
  

   the 
  box 
  with 
  water 
  and 
  put 
  in 
  enough 
  green 
  wheat 
  or 
  corn 
  to 
  cover 
  the 
  bot- 
  

   tom. 
  Scatter 
  the 
  infected 
  bugs 
  over 
  the 
  bottom 
  and 
  put 
  in 
  a 
  large 
  quantity 
  of 
  

   live 
  bugs 
  from 
  the 
  field 
  ; 
  a 
  quart 
  would 
  not 
  be 
  too 
  many 
  for 
  a 
  box 
  of 
  the 
  size 
  

   above 
  suggested. 
  A 
  smaller 
  box 
  may 
  be 
  used 
  for 
  a 
  smaller 
  quantity 
  of 
  bugs. 
  

   After 
  the 
  bugs 
  have 
  remained 
  in 
  the 
  box 
  two 
  days, 
  remove 
  half 
  of 
  them, 
  alive 
  

   and 
  dead, 
  and 
  scatter 
  them 
  over 
  that 
  part 
  of 
  the 
  field 
  where 
  the 
  bugs 
  are 
  

   thickest 
  ; 
  at 
  the 
  same 
  time 
  replenish 
  the 
  infection 
  box 
  with 
  more 
  bugs 
  from 
  

   the 
  field. 
  Continue 
  to 
  scatter 
  bugs 
  from 
  the 
  infection 
  box 
  over 
  the 
  field 
  at 
  

   intervals 
  of 
  two 
  days, 
  until 
  it 
  is 
  seen 
  that 
  the 
  bugs 
  are 
  dying 
  rapidly 
  all 
  over 
  

   the 
  field. 
  Keep 
  the 
  box 
  moist 
  by 
  repeated 
  sprinkling 
  and 
  change 
  the 
  green 
  

   food 
  as 
  often 
  as 
  it 
  loses 
  its 
  freshness. 
  

  

  Careful 
  attention 
  to 
  these 
  directions 
  will 
  often 
  insure 
  success 
  where 
  care- 
  

   less 
  use 
  of 
  the 
  infection 
  would 
  fail. 
  Make 
  daily 
  notes 
  on 
  the 
  appearance 
  of 
  

   the 
  bugs 
  in 
  the 
  infection 
  box 
  and 
  in 
  the 
  field, 
  and 
  of 
  the 
  weather 
  while 
  the 
  

   field 
  infection 
  is 
  in 
  progress. 
  Note 
  carefully 
  the 
  chinch-bug 
  conditions 
  in 
  

   neighboring 
  fields. 
  Keep 
  a 
  list 
  of 
  farmers 
  who 
  get 
  infected 
  bugs 
  from 
  your 
  

   farm. 
  

  

  Save 
  a 
  quantity 
  of 
  fungus-covered 
  and 
  non-fungus-covered 
  dead 
  bugs 
  in 
  a 
  

  

  (220) 
  

  

  