On the Chemistry of Light Production in Luminous Organisms. 109 
TaBLE 2.—Properties of photogenic substances. 
Property. Luciferase. Luciferin. 
| ee ence nner ere ee NSE See 
Salting out— 
By saturation NaCl......-.---++-- Not precipitated......... Not precipitated. 
By half-saturation IMieSO@ae 2-1 eer 1D See mois c mcroi ke Do. 
By saturation MgSOg.....------+- Nearly completely pre- Partially precipitated. 
cipitated. 
By saturation MgSO, tacetic acid..|.......+..2e2eseeeeeeess Do. 
By half-saturation (NHa)2SOz....-- Slightly precipitated. .... Not precipitated. 
By saturation (NH4)2SO4...-.----- Completely precipitated. . Nearly completely pre- 
cipitated. 
By saturation (NHy)2SO¢+acetic |...-..- 20. sere e eee eeee: Do. 
acid. 
Solubility in— 
Methyl alcohol........-----+++++> Insolubles..ciaccic-kr-t Soluble. 
Ethyl alcohol.........---++s--ee:[eeeee: TD Yee ¢ eee ecnchcee co ac Do. 
90 per cent.......--|.-+-+-- WOk cies ose ei Do. 
70 per cent.........-|.-----Do pine Bis Ss te ee tere Do. 
50 per cent.......-- Slightly soluble.......... Do. 
Propyl] alcohol........--++-++-++5> Imeolublesee cs cise ar si Do. 
Isobutyl alcohol.........-+--++++[e eee: WOka ae owo wees Fairly soluble. 
Amyl alcohol.........--:eseseess[oerees 1D Yap Otic Sido, O.0 aro Slightly soluble. 
Benzyl alcohol.........-----seee¢[eere WOR chow crow eines Soluble. 
INGEST He bo DCRR Ome UIE IoI OOo Dl loko Gano 1D Loy nee ee iececien ic Sic Fairly soluble. 
90 per cent.......-.-----|-+---: WD Gotan. c ces Meee Soluble. 
70 per cent.......----+-> Slightly soluble........-- Do. 
50 per cent........-..--- Fairly soluble.........-- Do. 
Ethyl acetate...........--s+eeee Insolublesoccecn ae ee 0. 
propionate.......-----eeeecfeeeees Wow asietieeeeesies Fairly soluble. 
butyratenecericier acer = clin “il siicnn IDE amo oacoenooe Do. 
MALOU AGC oc dene isa 21.010 1s o's lo el | eorousrs 5 WD) OMe na rictelane overeiek Slightly soluble. 
ATES do Roe Soto oeool |p moor WO sae eteiste serorenes Very slightly soluble. 
Glycerine... oc0ct- - se one che pe rena Oo eke ores Ge Oxebsich- Soluble. 
MED ysis Sc wade > aot ane vecenenre rede le [6'e rele ee IDO a eee RCO Do. 
TOU hatoes - oe oudiG oan cumin ooo |S ocn os 1DINa ets Sau soaked oe Insoluble. 
Ghloroform:. ..<- 6 .2c. 0220 = 2a elle mee ee DIDS Gmonancegadme Do. 
Carbon disulphide. ........---+-+-}erree: DOs ae eet tion Do. 
tetrachloride. ........--+-]-seee> DO ne oto noose Do. 
leaiillas codons oe Ode onmccom@bm oo. |aicamicr DOs soa nererseseste = Do 
Rill sent n oicte eile ioe tere sersvelis avons) lereksie) Iie IDs Ga eceeicne oat Do 
Belg) se reetescs ns ianesanntn ative c Stehe to siiene'ieys)fpersi= Sirs 1D yer, oe Ore Cotorconc Do. 
Petroleum ether........-.--++--ees[eeeee? iDYme goo wo aopE ode Do 
JNtItith, © Opa e Ba cae oeod 0 og obo mad| | manor AD Oe eesecte ensues exer Do. 
Glacial acetic acid.........---2++s|-eree> IDA so daogsecoo oC Fairly soluble. 
