[13] ril'IiliailEMBEY'OGRAPHTrCJOBErrOSBMOlTS Fl&'HESlSOini.a B^ 



iug between the vfesicles:.^as' the onlyipT9riron> wlrieh^wotiklj3stainvthJe 

 vesicles remaining perfectly transparent.' From this it.^!S'ds< eonctnded 

 that the contents of the A'esicles were not iia'otoplasmic or ntielear, biat 

 some iaidifferent flnSdi When imoiei'sed tin:' aieoliol or ether «i)f'dhffiage 

 was noticed^ so that it Waaihijgkly impioobaiMe that^fehe v€sicErl?w?i^ontebts 

 wereoily.^' .1 ';'••;!!: >>;.( -.i )h-*'/ -!■?; J rrf:' .-.^'i oJ -.'^ ,->';-/n ■:.;;'j^ ftrj-! >? 

 . lEAs has observed that the oil globule ■ of thei sateou-'eg'^ slf e!-8«r- 

 rdundedr by an albuminous envelope 5 this is probably. dU[ly«j* continiia- 

 tion of the protoplasm of the germinal^ layei-y as th<i oil ' always hsfsia 

 superficial position in these forms and is il# d(?.eply'imbeddednin;tihe 

 yelk, as m Brosmi'm, Cybhtm, Parcjihippus), i^nd'Elaoate. M€i40hemii^s 

 also shown that His is wrong' in his belief that the rose-coloredoilicS!'the 

 salmon egg is composed of lecithin, but is an oil not c<?agulable -at 

 100° C. nor under the iullnenec of concentrated acids, iusolubte in/ ajj^"<^^, 

 very soluble in ether amlT^lcohol, and i^ in^adably reawv^ed froia! sq^a 

 preserveil in a strong solution of the latter. If tht» egg has 'bem- p<Fe 

 viously hardenetl in chromic acid, andsectionsi are jtiepurfid,!]! l^d ^it 

 the places'formerly oetupied; byttheioil;sphfeiesiai*e'sh^ 

 openings around the edge of •■ the section ^ iiext the yelk envelopew ". The 

 oil is almost instantly blackened by osuiic aekl; in th«'.mif hanged state 

 it swims upon thesiirface oftU.e watePrwheh aifreshMeggi^f eyU^ aijd 

 tli!e;©ilfatowed>tO'eScape.[ .o'rj-dqa iio^ ojii io fitnitina nib ito notmmq 

 • ! :<> • j;v/ jx>ijii :ut'i .'i-j.-ni snh r- yd oao^Twa hii no %!i?inyqo Ayjlilvuhno 



Tile oT)set%'^er sought in Vaiil in these sthiined prepan'itioiis of the ripe 

 unimpregllated eggs for the germinative ' vesicle, and e<'iually fruitless 

 were his ehdeavors' to di'scove^r' this situ^tftt^ ihito'eys'ed iii THe^^ 

 neath the germinal disk after flio latter had been forined. *Tt r.sbeT[fn^ed, 

 therefore, that it has been broken u[) beffrre the egg 'hals- escaped from 

 its fdlliciein'tHfe &^ff^ -ihH tli^it' it^ r^iiiiihk-'Hyt^ rearrfeii^Mlh^Mserves 

 in some wdy in"doiiWe6tt6h Mth tlie' g 



the germinative vesicle towards the periphery of 'the'Ggg wasf eviif db- 

 sefved iii tb6 iMtoatUrd '^^g, ot-iti'thbke'itealfi^^ ft^^'Mr- 



mised that th'e' process of bre<^Mug iip talife place 'i^i^itW^compa^c^^^^ 

 rapidity. Kupfler's search f(t>r the germinative tesicle df the ovtftn bf 

 the herriiig was, according t6-'M^'%WTh 'fwjC^Ht^'as frilitTes^s'''^ 

 with the cod's egg. Like th^ oM^df teds;;those?of' fishes ^ai-ttia^e 

 their gerininative Vesicle before they leave the follicle 'iii' wMohijBfey 

 were developed. It is a most re matrk able fact that in some types, iiibl- 

 lusks, e. g:, the germinative vesicle— egg miCleus— should persist iw a 

 central positiofi after miittiration; ahtl^n'd^h^^, ii^lfdi 'e±kh^qMmie 

 ova of Elasmobranchs, Teleosts, and Aves, it disai)peai'H or i^ inetiilDS<^r- 

 phoSed by'tUe tiine of 'matiii'it;t' iitid befoi^e'the eg-g 1ms left its pfirent 

 follicle. ~Again~it: is equally' fe^r^ibTe. 'tlat 4ii::sm]p. forms the pfar 

 cells are developed iudependeutly of ijuprGgna,%%.w^ in others the 

 nuclear metamorphosis attendant upon the development of the polar 



