NO. 1451. BRAINS AND BRAIN PRESERVATIVES— HRDLICKA. 297 



EIGHTY PARTS OF 95 PER CENT ALCOHOL AND 20 PARTS 5 PER CENT 

 SOLUTION OF FORMALIN. 



(Alcohol, 80; water, 19; formalin, 1.) 



End of first week: All the brains in the 3 c. c. to the gram solution 

 had lost in weight; the average loss was 11.5 per cent, or one-ninth of 

 the original. Variation: From 85.27 (original weight 112 grams) to 

 91.45 per cent (original weight 117 grams). The heaviest brain lost 

 least. The specimen in the 6 c. c. to the gram solution (original 

 weight 106 grams) lost more than any of the above (15.1 per cent), 

 that weighed daily (original weight 109 grams) lost 11.47 per cent. 



End of one month: The solution was not changed at the end of the 

 first week nor at the end of the first month, except with the specimen 

 weighed daily. No especial difierence appeared in the results. All 

 the brains in the 3 c. c. to the gram solution lost slightly in weight 

 after the end of the first week (—0.77 to —2.09 per cent). Variation: 

 P'rom 83.48 to 89.31 per cent =5.83 per cent. The changes were not 

 harmonious with those of the first week nor proportionate to the 

 weight of the brains. The specimen in the 6 c. c. to the gram solution 

 lost 1.66 per cent, that weighed dailj- 1.56 per cent. 



Changes in the brain weighed dail}^: A moderate loss in weight 

 occurred during each of the first five da\'s, after which there was a 

 slow, continuous loss up to the end of the month and beyond. Neither 

 the first nor the second change of solution produced any efl'ect. 



SIXTY-FIVE PARTS 95 PER CENT ALCOHOL AND 35 PARTS 3 PER CENT 



FORMALIN. 



(Alcohol, 65; water, 34; formalin, 1.) 



End of first week: Weight of brains in 3 c. c. to the gram solution 

 had fallen in average nearly 4 per cent. The lighter brain lost some- 

 what more than the heavier one. The specimen in the 6 c. c. to the 

 gram solution lost much more than either of the above; that weighed 

 daily lost slightly more than either of the other two in similar quantity 

 of the preservative. 



End of first month: Weight of the two brains in 3 c. c. to the gram 

 solution had diminished but slightly, that of the specimen in 6 c. c. 

 to the gram solution distinctly more, while that of the brain weighed 

 daily was equal. 



Changes in the brain weighed daily: The first day a slight (1.56 per 

 cent) rise, then a gradual loss; an insignificant rise the first and third 

 days after the first change of solution, then stabilit}', with slight ups 

 and downs. Mo rise or fall in weight after the one-month's change of 

 the preservative. 



