92 REPORT OF THE COMMISSIONER OF FISH AND FISHERIES. 



SroRori-ASM 



Tliis was noted (but apparently res'ai'dcd as a third capsule) by Miil- 

 ler,^ and it appears iuseveral of his ligures. Subseipieiitly Lieberkiihn '^ 

 observed its exit from the spore and its amoeboid movements. He also 

 notes its visibility within thc^ spore.^ These observations have been 

 conlirmed bj^ Balbiani^ and later by others (see j^p. 203,287). 



The si>oroplasm is extremely transparent, more or less granular, and 

 contains nuclei (1 or more), sometimes a vacuole, and, at any rate in 

 the genus ^fijxoholus, a variable number of brightly refriugent granules. 



IfuclcL — These were first demonstrated by Thelohan.' Their number 

 is variable in the same spore, according to the stage of development. 

 In Mi/.whoh(s ellipsoidcs, Thelohau demonstrated their origin by contin- 

 uous division from a primitive single one. He further says " that all 

 species studied by him (with the possible exception of the GJugcn species, 

 in which the small size of the spore prevented accurate determination) 

 have shown 2 nuclei. This accords Avith my own observati(nis. 



Granules ("refringent globules," etc.). — These have been noticed in 

 several Myxoholus species. They are described under that genus (see 

 p. 209). 



Vacuole. — This structure is of two types: (1) The noniodinestaiiiing 

 {aniodinopMle) vacuole. This is known only in, and forms a marked 

 characteristic of, the Crijpfofi/sfes. It is situated in the large extremity 

 of the ovoid or pyriform spores and is unaffected by iodine. This 

 structure was first observed, but not at that time recognized as a 

 vacuole, by Thelohan." Subsequently he recognized its true nature." 

 (2) The iodine-staining {iodinophile) vacuole. This is known only in, 

 and forms a marked characteristic of, the Myxoholidm. It is stained by 

 iodine dark brown against a light yellow-brown ground. This reaction 

 is best obtained with a dilute solution (aqueous, with potassium iodide). 

 Further details are given under Myxoholus (p. 208). 



' Miillcr's Archiv., 1841, p. 484, pi. 16, fig. 3 i, k; cf. fig. 5. 



^Miillcr's Archiv., 1854, pp. 353-4, pi. 14, figs. 7, 8. 



3 Bull. Acad. Roy. ]5elg., 1854, xxi, pt. 2, p. 21. 



••Comi)!. Rend. Acad. Sci. Paris, 1863, i.vii, p. 160. 



'^Compt. Rend. Acad. Sci. Paris, 1889, cix, pp. 920-21. For Perugia's confirmation, 

 see Myxoholus / merlitcii (p. 242). For Blitschli's "nucleus", see j). 219. 



«Conipt. Rend. Acad. Sci. Paris, 1892, cxv, p. 1092. 



' Aiinal. de Microgr., 1890, ii, p. 211, pi. 1, fig. 17fl, h. 



^Relative to the houujlogy of the vacuole, Th(51ohan says: 



" Is there any connection hetweeu the central vesicle and the rest of segmentation 

 of the other ><poro:oa ? A certain fact is that the aspect of the plasmic mass of the 

 spores of the MyxosporhUa with that vesicle refi-actory to staining, and the nuclei 

 disseminated in the protoplasm, recalls in a striking manner certain phases of devel- 

 opment of the spores of the Gregarines." 



