268 REPORT OF THr; COMMISSIONKR oF FISH AND FISHKKIES. 



liistov.v than the migration of the iilasniatic; miss usually described. The presence 

 of many empty lapsuh^s ' in the sections would lend weight to this view of the 

 expulsion of the nontents of the spore, and in lig. 4a I have represented a capsule' 

 with a single pole corpuscle, whidi apjx'arcd to bo in the act of csca]iing througli a 

 rent in the capsule. 



Filaments best seen in sections, stained with Babes's anilin-water 

 safranin where they stain i)roininently yellow; lenoth varying consid- 

 erably, many oecnrriiig- curled up at the end as though only partly 

 unwound, measuring when fully projected to 8 times the spore-breadth, 

 extending far into the surrounding tissues; sometimes dimly visible 

 through capsular wall; extruded parallel to the shorter (anteroposte- 

 rior) diameter of the spore, 



Sporoplasm varying considerably in size and shape, and sometimes 

 filling all tlie extra-capsular portion of the shell cavity; in this con- 

 dition presenting no evidence of segmentation. In other cases less 

 extensive, being sometimes very small and shrunken,- the sporoplasm 

 then frequently showing a well-delined segmentation, the line of division 

 extending through its middle |i^ e., coinciding witli the vertical plane j. 

 Each si)oroplasm-half envelops, in the form of a well-defined crescent, 

 the corresponding capsule. Nonvacuolate (letter to author, 1893). 



The sporophu;m stains with Pfitzner's alcoholic safranin a light i)iiik- 

 ish hue. appearing under a Leitz j\r in anilin-stained sections, delicately 

 granular; no other structure discernible. Nucleus and evidence of 

 nuclear contents invariably absent. (Jhlmacher adds: 



I could not even dcuioustrate the micrococci-likc particles in (he plasmatic body, 

 as have been described by Lutz, or the sairauophile particles of Jiiitsclili. 



IMicro-chemistry: Ohlmac^her finds the sporoi)lasm constantly cyan- 

 ophilous, the capsules constantly erythrophilous. This occurs with 

 carbolic fuchsin and carbolii*- iodine green (liussell's method); the 

 capsules staining a brilliant red, the sporo[)lasm light green. The tint 

 of the sporoplasm (consecjuently also the degree of dicliromopiiilisni) 

 varies from violet to a well-defined green. This difference depends in 

 large part on the developmental stage of the sponqdasm. Wherelarge 

 and unseginented and occupying a large part of the shell cavity the 

 green stain was less clearly defined; where more condensed and'divided 

 into the 2 crescents closely ap]died to the capsules, the green was well 

 marked. A striking dilTerentiation is produced by Pfitzner's alcoholic 

 safranin, followed by aque(nis methyl blue, rapid washing in alcoliol, 

 and clearing in xylol. The Biondi-Heidenhain triple stain and Wat- 

 ase's cyan.in-ehromatrop failed, a result attributed to uonpenetration 

 of the shell by the stain. On the other haiul, the success of fuchsin- 

 iodine-green and safranin-methyl-blue seems, Ohimacher says, to be 

 due solely to their more powerful staining projjerties, which permit them 

 to penetrate the somewhat resi.staiit shell. 



This dichromoi)hilism of the capsule and sporoplasm Ohbnaeher eoni- 



' By this term he means the spore-sliell. 

 -Due. T think, lo absujnte alcohol fixation. 



