272 REPORT OF TIIK COMMISSIONER OF FISTI AND FISHERIES. 



Micro-chcminlry. — The ]);nasitos were studicfl fresh (l)y teasini;- kidney tissue, anil 

 examiuiiig this in a liaiiying drop, or in iluid media of ditfevent kinds), and also 

 after treatment with various tixing and staining agents. In the fresh state, a dilute 

 solution of potassium hydrate caused a swelling of the spure, and brought out the 

 shell and tilauients plainly. Glycerin acts well as a medium for the examination of 

 the fresh spore. Probably the best medium to use for the hanging drop is toad's urine. 

 Iodine (a<|ueou8 solution) colors the spore a uniform brown. In tixing cover-glass 

 prejiarations, no advantage was gained by fixing them in alcohol and ether, or in 

 osmic acid, over that obtained by passing tlie covers through a llame. In the fresh 



state the lihinients were made jdainer in fixed cover-glass preparations 

 [Page Gt)2.] by a number of reagents. Acjueous methyl blue and Babes' anilin 



water safraiiin bring the filaments into view quite satisfactorily. 

 As fixing agents, Flemiuiug's solution, Keidenhain's mercuric chloride solution, 

 absolute alcohol, Caruoy's acetic alcohol, and Perenyi's fluid were tried, the first and 

 last being found unsuitable on account of the production of shrinkage and distor- 

 tion. The fixed material was imbedded in xylol paraffin by the usual methods. 

 Numerous separate and combined stains were cmph)yed with varying results, the cap- 

 sules with almost all stains showing the greatest affinity for the coloring nuitter, the 

 de<n-ee of alifinity varying son\c\vhat in dill'erent spores. Pfitzner's safranin is espe- 

 cially good, with a striking affinity for the capsules. Ohlmachor's dichroniopbilism 

 was demonstrated with fuclisin and iodine green (Russell's method), and with 

 safranin and methyl blue (Oblmacher's method). "This chroinophilous reaction is a 

 very striking and possibly significant phenomenon in these organisms." 



90. Chlororayxnm (SphBcrospora) perlatum Gnrley, 1893. PL -10, fig. 2. 



(Psorosperm of Acerina ccrniia, Balbiani, 1883, Journ. de Microgr., Vii, pp. 201, 

 204, lig. 44; ib., Balbiani, 1884, Lemons sur Ics Sporozoaires, p. 133, fig. 40.) 



Myxoholns jm-laliis, Bull. U. S. Fish Cora, for.1891, xi, p. 415; ib., Braun, 1894, 

 Centralbl. f. llakt. u. Parasitenkde, xv, j). 87. 



No description (sec also p. 205). 

 Habitat. — On Acerina ctniua L. 



91. Chloromyxum (Spliaerospcra?) sp. incert. PI. 40, fig. 3. 



Spore of Lota vulgaris, Butschli, 1882, Bronn's Thier-Keich., i, pl.38, fig. 22. 

 • Cyst unknown. 



MyxoHporidium. — Not described. Tlie sporoblast produces a single 

 spore ? ' 



kSporc. — Not described. For the reasons given on p. 205, the present 

 generic reference of this species is probably the correct one, and the 

 species should be closely comj^ared with C. mucronatum. 



Habitat. — Ovary of Lota lota L. (= vulgaris); ling. 



' " Each spore in a special transparent membrane." 



