124 American Fisheries Society 



Put back in tar solution 9:15 A. M., October 30. Again removed 

 to water containing no tar at 1:20 P. M. Continued to swim on 

 back and dart occasionally. Put back in tar solution November 

 2, 5:00 P. M. Dead November 3, 6:00 P. M. 



Experiment 5907-3. 



No. of fish: 1. 



Used: Trace* of tar No. 5907; 1500 cc. water. 



Put in solution 5:37 P. M., March 2, 1915. March 13, solu- 

 tion renewed; fish sick. March 14, swimming on back, 1:00 P. M. 

 March 15, 9:00 A. M., nearly dead. March 15, 6:00 P. M., dead. 

 The fish showed signs of paralysis just prior to death. 



"No. 5962 is said to be a product obtained as sludge in treating 

 certain petroleum distillates with alkali after the sulphuric acid 

 treatment. The sample shows 2.43 per cent of alkaline ash upon 

 ignition. The presence of this alkali renders the material readily 

 miscible with water in the form of an emulsion, although this 

 emulsifying character is lost to some extent when the material has 

 been exposed to the atmospheric influences for some time. The 

 product is obtained in the refining of Texas petroleum." 



Fish subjected to water to which traces of the tar had been 

 added showed no effect after 19 days when the experiment was 

 discontinued. 66 parts : 1,000,000 of water caused death in about 

 36 hours. 13 parts : 1,000,000 of water caused death in about 

 three days. 



"No. 6122 is said to have been produced by dissolving about 

 40 per cent oil asphalt in some high boiling petroleum distillate. 

 It was presumably prepared from products obtained from Illinois 

 crude petroleum." 



66 parts : 1,000,000 of water caused death in about three days. 



13 parts : 1,000,000 of water caused death in about three days. 



Trace of tar had no effect in 19 days when the experiment was 

 discontinued. 



"No. 6550 is a Mexican crude petroleum having a flash point 

 of 26° C, and a burning point of 53° C. It has a loss of 27.68 

 per cent in the standard volitization test and, through the loss of 

 lighter materials contained in it, sets up quite rapidly when 

 applied to a road surface." 



* A trace of tar was obtained by dipping a match stem into the tar and 

 then into the water. A thin, irridescent film on the surface could be seen. 



