ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 233 



ratus was originally devised for keeping diphtheria bacilli alive, but it 

 was found that the broth in the flask soon became highly virulent 

 owing to diffusion from the sac, and that bacteria remain alive much 

 longer in this apparatus than had been anticipated. 



Gage, Stephen De M.— The Bacteriolysis of Peptones and Nitrates. 



[Deals with the biochemistry of sewage purification.] 



Technology Quarterly, xviii. (1905) pp. 5-39. 



(2) Preparing- Objects. 



Demonstrating the Structure of Corals* — F. Menneking, in his 

 research on certain Corals, used material which had been preserved in 

 spirit and decalcified with sulphuric acid. The material was then 

 hardened in graded alcohols and imbedded in paraffin. The sections 

 were stained with hasmalum. 



Besides the ordinary paraffin imbedding, the author also adopted 

 Schcenemann's method f for bone, with good results. 



Trichloracetic Acid as a Fixative .% — M. Heidenhain recommends 

 the use of trichloracetic acid for fixing tissue. From an experience of 

 ten years he has found its action very satisfactory in from 5-10 p.c. 

 solution. Its one defect is that it makes connective tissue swell up, 

 but this inconvenience is obviated by after-treatment with absolute 

 alcohol, which should be frequently changed and allowed to act for a 

 considerable period. 



Presence of Negri's Bodies in Rabies.§ — A. Bongiovanni obtained 

 negative results when experimenting on rabbits with fixed virus ; in the 

 controls with street virus Negri's bodies were always present. The parts 

 examined were cornu ammonis, cerebellum, Gasserian and spinal ganglia. 

 The pieces were fixed in Zenker's fluid, and the sections stained by the 

 methods of Fasoli,|| of Mann, with Ehrlich's acid-hsematoxylin, and with 

 Heidenhain's iron-hsematoxylin. 



Part of the research was to ascertain the effect of radium. The 

 results show that the action of the rays delayed the activity of the virus 

 and postponed the inevitable termination. 



Demonstrating the Phenomena of Maturation in Oogenesis and 

 Spermatogenesis.^! — P. Lerat used Cyclops strenuus only in his re- 

 searches, obtaining the specimens chiefly from pools and puddles. The 

 water was filtered, and after the removal of such animals and plants as 

 could be descried, the Cyclops were found in the stem of the funnel. 

 These were killed by immersion in Grilson's fluid for about 10 minutes 

 and afterwards washed in water for about half-an-hour. Cyclops strenuus 

 was then picked out under a Microscope. Fixation and imbedding were 

 performed in test tubes, a procedure which lent itself to an easy change 



* Archiv Natur., lxxi. (1905) p. 246. 

 t See this Journal, 1903, pp, 107 and 371. 

 \ Zeitschr. wiss. Mikrosk., xxii. (1905) pp. 321-4. 

 § Atti R. Accad. Lincei. , xiv. (1905) pp. 454-62. 

 jl See this Journal, 1905, p. 386. 

 t La Cellule, xxii. (1905) pp. 163-98 (4 pis.). 



