ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 



239 



razor has an almost straight movement, a great advantage for sectioning 

 delicate objects. The direction of the knife movement is indicated by 



Fig. 35. 



the dotted lines (fig. ?>b). The instrument (fig. 34), like that shown 

 in the December Journal, 1905, p. 766, has a handle and a heavy base. 



Preparing Liver for Demonstrating Hepatic Ferments. * — E. W. 

 Carlier killed the animals (white rats) with coal-gas, and then washed 

 out the blood vascular system very thoroughly with normal saline 

 solution. The injection vessel was then filled with picro-corrosive 

 formalin warmed to body temperature, and about half a litre passed 

 through the animal. When the animal was cold the liver was dissected 

 out, cut up into small pieces, placed in graded alcohols, beginning at 

 50 p.c. to chloroform and paraffin. 



The most useful staining methods were Mann's methyl-blue-eosin, 

 toluidin-blue-eosin, MacAllum's method for unmasking albuminoid iron, 

 and Heidenhain's iron-alum-haematoxylin. The sections were cleared 

 in inspissated turpentine and mounted in turpentine balsam. 



(4) Staining 1 and Injecting'. 



Demonstration of the Flagella of Motile Bacteria.f — E. W. Duck- 

 wall, after describing the necessary refinements he employs in the making 

 of suitable cultures, according to the variety of motile organism under 



* La Cellule, xxii. (1905) pp. 431 56 (17 figs.). 



t Centralbl. Bakt., l*e Abt., Ref., xxxvii. (1905) p. 360, 



