382 SUMMARY OF CURRENT RESEARCHES RELATING TO 



Slide-Basket for Staining Twelve Sections Simultaneously.* — 

 Kjer-Petersen mentions a basket (fig. 52) which he has had constructed 

 for the purpose of facilitating the staining, etc., of 

 films of tuberculous sputum. Though the method 

 of using the apparatus is obvious, no directions are 

 given, and no details as to its construction. 



Staining Bacillus typhosus in tissues.t — P. Foa 

 fixes the pieces in the following fluid : — sublimate 

 2 grin., Muller's fluid 100 grin., followed by alcohol, 

 and then stains with a mixture of methyl-green and 

 pyronin (Pappenheim's formula). In about 5 minutes 

 the bacilli are stained deep red. 



Modification of Flemming's Triple Stain.! — V. 

 Bonney has devised the following easy process of triple 

 staining for cytological and histological purposes. It 

 Fig. 52. is based on the method of Flemming, a method which 



has received praise and blame. The pieces, which 

 should be small, are fixed in acetic alcohol (glacial acetic acid 1 part, 

 absolute alcohol 2 parts, or in Hermann's or Flernming's fluid. Imbed, 

 and section in the usual way. Stain for 1 hour in saturated aqueous 

 solution of safranin. Wash in water. Stain for a quarter of an hour in 

 an aqueous saturated solution of methyl-violet. Wash in water, and wipe 

 the slide dry, except the part occupied by the section. Flood the sHde 

 with the following solution : To 20 c.cm. of acetone add drop by drop 

 a saturated aqueous solution of orange Gr. until the precipitate which 

 appears on shaking is just dissolved in excess of the aqueous solution, 

 then filter. Kun off the fluid and flood again with the same solution, 

 and when the section has assumed a faint brownish-pink colour, pour off 

 the orange-acetone solution. Wash in acetone for a few seconds. Wash 

 in xylol. Examine under a low power to see if the proper result has 

 been attained. Then wash in two fresh changes of xylol. Mount in 

 xylol-balsam. 



Chromatic elements stain a rich violet ; spindle fibres of mitosis a 

 faint pink ; cytoplasm a rose pink ; intercellular tissue a pale yellow. 



(5) Mounting-, including- Slides, Preservative Fluids, &c. 



Demonstrating Pollen Grain Variation^ — J. B. Pollock placed 

 anthers, which were almost dehiscing, in Kleinenberg's hasmatoxylin for 

 24 hours. They were then washed in alcohol, and changed very gradually 

 from 96 p.c. alcohol to clove oil. In this medium the pollen sacs were 

 broken up, and the pollen grains thus set free were examined by mounting 

 a drop of the oil containing them. 



Method for making Permanent Preparations of Amyloid Degenera- 

 tion. || — P. Meyer finds that permanent preparations of tissues affected 



* Centralbl. Bakt., 2*e Abt., xvi. (1906) pp. 191-2 (1 fig.). 



t Giorn. R. Accad. Med. Torino, 1905, Nos. 5-6. See also Centralbl. Bakt., 

 lte Abt. Ref., xxxviii. (1906) p. 50. J Lancet, 1906, i. p. 221. 



§ American Naturalist, xl. (1906) pp. 253-86 (16 figs.). 

 || Vircbow's Arcbiv, clxxx. (1905) pp. 359-61. 



