ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 617 



aqueous), 75 c.cm. distilled water, 50 c.cm. absolute alcohol. Several 

 other staining methods were tried, among which may be mentioned the 

 vital staining with methylen-blue followed by fixation with ammonium 

 molybdate. 



Demonstrating the Lymphatic Vessels of the Prostate.* — 

 R. Caminiti, after recounting the methods of previous investigators, 

 describes the procedure which has given the best results. After tying 

 the ureters and urethra with silk thread, a ■ 5-1 p.c. aqueous solution of 

 silver nitrate is injected into the gland by means of a Pravaz syringe. 

 Injections are made in numerous places and in all parts. The gland is 

 then washed in distilled water, and afterwards placed in absolute 

 alcohol, frequently changed, until it is sufficiently hardened. The 

 sections (freehand or paraffin) are exposed for a few minutes to sunlight, 

 and then transferred to a weak alcoholic solution of sodium hypo- 

 sulphite (0*5-1 p.c). After repeated washing in absolute alcohol, they 

 are placed in bergarnot-oil for some hours, and thence transferred to 

 xylol and balsam. If the sections be over-blackened by the sun, they 

 should be placed in 3-5 p.c. potassium iodide solution in 1)5 p.c. alcohol. 

 From this they are transferred to 95 p.c. alcohol, and then to sodium 

 hyposulphite. 



In some cases Berlin blue was also injected through the aorta or 

 iliac artery before the silver nitrate was used. 



Investigating the Structure of Spinal Cord of Macaque Monkey.f 

 Mabel P. Fitzgerald injected this monkey (Macacus sink us) by Mann's 

 method with picro-corrosive-formaldehyde solution. 2 ' 5 grm. of sub- 

 limate were dissolved in boiling water, and then 1 grm. of picric acid 

 added. When cold, and just before use, 10 c.cm. of formol were added. 

 The cord was removed after about 24 hours, and then, together with 

 the spinal ganglia, placed in 50 p.c. alcohol. It was next dehydrated in 

 up-graded alcohols, and imbedded in paraffin (m.p. 52°). Serial sections, 

 20-20 /a thick, were cut, and, having been treated in the usual way to 

 remove the mercury, were stained with eosin and toluidin-blue. 



Studying the Structure of Visceral Ganglion of Anodonta.J- — 

 T. Freidenfelt first injected the fresh material with methylen-blue of 

 various strengths, and after a preliminary examination to ascertain if 

 the staining had been successful, fixed in Bethe's fluid. After harden- 

 ing, the material was imbedded in soft paraffin, and coarse sections 

 made. Good results were obtained by Lavdowsky's method, which 

 consists in mixing fresh egg-albumen with about a similar quantity of 

 1 p.c. methylen-blue in physiological salt solution. Still better was to 

 take some of the animal's blood and mix with methylen-blue diluted with 

 ammonium chloride. The methylen-blue used was from 0*1-0 "5 p.c, 

 and the ammonium chloride about ' 1 p.c. 



The author remarks that Bethe's method of after-treatment with 

 potassium bichromate is not, in his opinion, altogether satisfactory. 



* Anat. Anzeig., xxix. (1906) pp. 172-85 (4 figs.). 



t Proc. Roy. Soc, Series B, lxxviii. (1906) pp. 88-144 (numerous charts and figs.). 



X Acta Univ. Lundensis, xl. (1904) received Aug. 1906, Afdeln 2, No. 5. 



Oct. 17th, 1906 2 S 



