■626 SUMMARY OF CURRENT RESEARCHES RELATING TO 



and 5 p.c. acetic acid 1 part, on a clean cover-glass. The size of the 

 drop must be small so that the film dries quickly, after which it is fixed 

 in the flame. The film is then stained hot with carbol-fuchsin, after 

 which it is decolorised with 1 p.c. H 2 S0 4 until the preparation is of a 

 pale rose colour. 



After thoroughly washing with water, the film is stained with 1 p.c. 

 aqueous methylen-blue, or malachite green, for 2 minutes. The washed 

 and dried film is mounted in balsam. A variation of the method may 

 be made by contrast-staining and decolorising at the same time with a 

 saturated solution of methylen-blue in 1 p.c. sulphuric acid. 



Demonstrating Negri's Corpuscles.* — Bohne removed from the 

 comu ammonis pieces 5— J mm. thick and placed them in pure aceton 

 at 37° for 30-45 minutes, or longer if the brain were much decomposed, 

 until they were firm. The pieces were then immersed in liquid paraffin 

 (m.p. 55°) and kept therein at 60° for 60-75 minutes. The sections 

 were removed to cold water to which some gum arabic had been added, 

 and when placed on the slide were dried at 60°. After getting rid of 

 the paraffin the sections Were stained for ^—4 minutes by Mann's method 

 (35 c.cm. 1 p.c. aqueous methylen-blue solution -(- 35 c.cm. 1 p.c. eosin 

 solution -f- 100 c.cm. distilled water). After washing in water and then 

 in water and alcohol the sections were treated for 15-20 seconds with 

 30 c.cm. absolute alcohol -4- 5 drops of 1 p.c. caustic soda solution. The 

 sections were then washed in absolute alcohol followed by tap-water 

 (1 min.) and then by water slightly acidulated with acetic acid, after 

 which they were rapidly dehydrated and mounted in balsam. 



The author regards the presence of Negri's bodies as diagnostic of 

 rabies, but expresses doubt as to their parasitic nature. 



Demonstrating the Striated Membrane in the Erythrocyte of 

 Salamander.f — F. Meves deposited thin films in a moist chamber for 

 various times (a few minutes to half-an-hour) and then fixed them with 

 weak Flemming's solution (1 p.c. chromic acid 25 c.cm., 1 p.c. osmic 

 acid 10 c.cm., 1 p.c. acetic acid 10 c.cm., distilled water 55 c.cm.) to 

 which 1 p.c. salt was added. After washing in running water the pre- 

 parations were stained with safranin and Delafield's hasmatoxylin, or 

 with Flemming's triple stain (safraniii-gentian-orange). In the former 

 case the films were treated for 24 hours with 1 p.c. aqueous solution of 

 safranin followed by neutral alcohol, and finally by the haeniatoxylin for 

 6-12 hours. In the latter case the films were treated according to 

 Flemming's directions, though the author differentiated for half-an-hour 

 with oil of cloves previous to mounting in balsam. 



Staining of Treponema pallidum Schaudinn.J — B. Galli-Talerio 

 recommends Giemsa's solution as supplied by Grubler. He uses solutions 

 of 1 : 10 and 1 : 20 acting from 5-20 hours. The Treponema is coloured 

 red, the bacteria and spirilla becoming deep violet. 



* Zeitschr. f. Hygiene u. Infekt., lii (1905) p. S7 (1 pi.). See also Centralbl. 

 Bakt. Kef., xxxviii. (1906) pp. 220-1. 



t Anat. Anzeig., xxviii. (1906) pp. 444-7 (2 figs.), 

 t Centralbl. Bakt., lte Abt. Orig., xli. (1906) p. 745. 



