628 SUMMARY OF QUERENT RESEARCHES RELATING TO 



eosin and 1 litre of 1 per thousand medicinal rnetkylen-blue. The pre- 

 cipitate is carefully filtered and washed with distilled water, and then 

 dissolved in 0'25 p.c. methyl-alcohol. The dried but unfixed films are 

 stained for 2 minutes and then treated with distilled water for 1 minute, 

 after which they are mopped up with blotting paper. The distilled water 

 should be perfectly neutral. 



A. Huisman * praises Jenner's stain, as it gives more reliable and 

 satisfactory results than other methods. He has devised the following 

 modification. He mixes equal parts 0*175 p.c. solution of solid azur- 

 blue in absolute methyl-alcohol, and 0*825 p.c. solution of eosin B. A. 

 Hochst in the same medium. 



The mode of using the stain is the same as in the original method. 



Staining Spirochaeta pallida.t— F. R. M. Berger finds Dahlia a 

 useful reagent for staining Spirochceta pallida {Treponema pallidum). 

 He dilutes 4 c.cm. saturated alcoholic Dahlia solution with 20 c.cm. 

 distilled water. 



Very thin films are fixed for 5-10 minutes in absolute alcohol and 

 then dried. The films are then treated with a few drops of Griemsa's 

 azur ii. solution for a minute. After washing in water they are dried 

 and passed through the flame. The films are next treated with a few 

 drops of the above described Dahlia solution for 2-3 minutes. After 

 this they are washed in tap-water, dried, rapidly passed through the 

 flame, and mounted in balsam. Instead of Dahlia, gentian- violet may 

 be used ; in this case the staining is somewhat darker. 



Balazsy, D. — Zur Glimmertechnik. 



[Gives a modification of Heidenhain's method of treating sections for class 

 purposes. See this Journal, mite, p. 110. 



Zeitschr. wiss. Mikrosk., xxiii. (1906) pp. 12-14. 



Prowazek, S. — Technik der Spirochate-Untersucliung. 



[A resume of the various methods employed for demonstrating the structure 

 of Treponema pallidum.'] Tom. cit„ pp. 1-12. 



(6) Miscellaneous. 



Modification of Schultze's Clearing Method.! — E. C. Hill, after 

 describing Schultze's, Tan Wijhe's, and Lundvall's methods for clearing 

 embryos and other anatomical specimens, details the procedure adopted 

 in the Johns Hopkins Anatomical Laboratory. 



The specimens are first injected with Indian ink diluted to one-third 

 its commercial strength. The addition of a small amount of weak 

 ammonia obviates precipitation of the medium. The injection of small 

 embryos should be carried out in warm water, with the membranes still 

 intact. All unnecessary tissue should then be removed. 



The specimens are next placed in 95 p.c. alcohol until completely 

 shrivelled (3-7 days). They are then removed to 1 p.c. KHO until 

 transparent (4-48 hours). After this they are placed in 20 p.c. glycerin 



* Med. et Hygiene, No. 4 (1906). See also Brit. Med. Journ. (1906) ii. Epit. 48. 

 t Miinchen. Med. Wochenschr., 1' 06, p. 1209. See also Zeitschr. wiss. Mikrosk. 

 xxiii. (1906) pp. 224-5. 



X Bull. Johns Hopkins Hosp., xvii. (1906) pp. 111-15. 



