74 



Journal oi Entomologj .mil Zoolog) 



paraffin of a melting point oi 60-62 degrees, but it was necessary 

 to peel ofl the cuticle and withdraw the setae oi those fixed in hot 

 mercuric chlorid. I he internal connective tissues, however, were 

 better preserved in this fluid, ["here seemed to be no marked dif- 

 ference in fixation oi the other tissues. 



Staining. The most effective stain for tracing out gross anatomy 

 and nerve distribution proved to be a faintrj acidified borax ear- 

 mine used after a fixation oi hot mercuric chlorid. 1 he muscular 

 and epithelial tissues were stained a uniform light pink, the nervous 



tissues a darker pink and the connective tissues, especially the peri- 

 neurium a bright red. Villain's copper hematoxylin, iron hema- 

 toxylin, mu\ double stains ot these and methylin blue with eosin, 

 "lieht grim" .\wd neutral red were ot value tor cellular detail. 



Reconstruction Method. A was .\nd blotting paper model ot the 

 brain (Fig. 2), was made in the usual way. lo supplement this 

 two stereographic reconstructions (Figs. 1. 4) were made which 

 were of ad\ antage o\ er the vn a\ model in the I ollow ing w a\ s. 1 he\ 

 are easier to make, eliminating the steps that involve cutting out. 

 impregnating and reassembling the parts ot the wax reconstruc- 

 tion. It is possible as was dime in Fig. 1 to make a "sciagraphic 

 reconstruction" that will show the internal parts in their normal 

 relation to the other organs, h necessary, colors could be used 

 to make the morphological differentiation clearer. When the re- 

 construction is finished it is the reproduction at a certain magnifica- 

 tion of am desired aspect ot the object and is equal in all import- 

 ant respects and superior in man\ respects to a photograph or draw- 

 ing ot' a wax reconstruction. What little distortion there is ma\ 

 be calculated as a function ot the displacement angle and easiK 

 I. iken into account. 



The method pursued may be described as follows: I he object 

 should be embedded with a piece of liver or similar tissue having a 

 smooth and quite rectangular face parallel to the axis ot the speci- 

 men. The pieces ma\ be arranged ow a watch crystal after infiltra- 

 tion and the embedding paraffin poured on hot enough not to form 

 a crystallization capsule around them. Ibis ones an orientation 

 guide whose projection is represented in b ( Fig. 14V Fig, 14 is a 



