174 
o 
Specimen 10265 ; October 11th, 1g00 ; Knysna Heads, N.E. 
3 E., 121 miles; 47 fathoms; bottom, rock; fixed in 3 per 
cent. formalin, and transferred to 70 per cent. alcohol. 
Specimen 13203; July 30th, Igor; near East London, 
Cove Rock, N.W. 3? W., 134 miles ; 80-130 fathoms; bottom, 
coral and rock ; fixed in 3 per cent. formalin, and transferred 
to 70 per cent. alcohol. 
Specimen 14273; February roth, 19602; Cape St. Francis; 
N.E. by E. 4 E., 36 miles ; 70 fathoms ; bottom, rock ; fixed 
in 3 per cent. formalin, and transferred to 70 per cent. alcohol. 
Specimen 18551; September 13th, 1904; Cape St. Blaize, 
N.W. by W., 5 miles; 31 fathoms; bottom, mud; fixed in 
74 per cent. formalin, and transferred to 70 per cent. alcohol. 
Specimen 185514 ; selected branches of the last in which 
the polypides were abundant ; fixed in osmic acid 1 per cent. 
solution for 5-10 minutes, washed for several hours in distilled 
water, and transferred through grades of alcohol up to 90 per 
cent. ‘ 
Specimen 186124; October 18th, 1904; Cape St. Blaize, 
W. by N., 5 miles; 29 fathoms ; bottom, fine sand ; procured 
by large trawl, and hauled up off Cape St. Blaize, N.N.W., 8 
miles; 38 fathoms; bottom, fine sand; fixed in glacial acetic 
acid for 15 minutes ; washed in 50 per cent. alcohol for 8 hours, 
and transferred to 70 per cent. alcohol. 
Specimen 18612B; material as above; fixed in absolute 
alcohol, 24 hours, and transferred to 70 per cent. alcohol. 
Specimen 18612c; material as above; fixed in fluid as 
follows : corrosive sublimate 7 grammes, distilled water 100 
cc., acetic acid 1 cc. ; after 15 minutes, washed in 70 per cent. 
alcohol, and transferred to more 70 per cent. alcohol. 
Specimen 18633pD ; October 20th, 1904; Cape St. Blaize, 
N., 5 miles; 34 fathoms; bottom, mud; procured by large 
trawl, and hauled up off Cape St. Blaize, N.E. 4 E., 6 miles ; 
30 fathoms ; bottom, mud ; fixed in equal parts of sea water 
and Flemming’s solution (5 per cent. acetic acid added to osmic 
acid and chromic acid solutions at time of using); after 24 
hours, removed to fresh water, frequently changed, for 2 
hours ; then 30 per cent. alcohol, 6 hours ; 50 per cent. a!cohol, 
g hours ; and finally 70 per cent. alcohol. 
Specimen 18633E ; material as above; fixed in Perenyi’s 
fluid, 6 hours ; washed in 70 per cent. alcohol, 24 hours ;trans- 
ferred to 70 per cent. alcohol. 
Specimen 18633F ; material as above ; fixed in glacial acetic 
acid, 20 minutes ; washed in 50 per cent. alcohol, and trans- 
ferred to 70 per cent. alcohol. 
