Puget Sound Marine Sta. Pub. 



V^oL. 1, No. 1 



To determine just where the growth takes place a number of exper- 

 iments were performed. For example the first two feet of two healthy 

 fronds were removed from the stipe, cut into three-inch sections, and 

 tacked by a single tack at the center of each piece, to a raft floating in 

 such a way that they were held about an inch under the surface of the 

 water. The ends were slipped under two narrow strips of cloth tied 

 around the board, which helped to hold them in place. Plate 1 will 

 explain the contrivance better. At the same time two feet of another 



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K 



mm-' 



PLATE I. 



a, pole about 4 inches in diameter ; b, wooden strips ; c, board 1 inch 

 thick ; d, strip of cloth ; e, short piece of lamina of Nereocystis; f, carpet 

 tacks ; g, long piece of lamina Nereocystis. 



frond was attached whole at one side of the short pieces. Table 3 shows 

 the results of growth. The increase in the two-foot piece was greater 

 than the added growth of the two feet of the three-inch pieces. This was 

 to be expected since a number of the growing cells would be destroyed 

 in cutting; also the "shock" to the plant would probably be greater and 

 not so quickly overcome. Some of these pieces were left until they had 

 reached more than double their length. Growth was found in each of the 

 pieces, even to the last one of the two feet. 



Table 3. Showing relative length in inches of 3-inch sections of fronds 



attached to raft. 



Dates 



July 8 I July 9 | July 11 | July 15 | July 20 



1st 3-inch piece 

 2nd 3-inch piece 

 3d 3-inch piece . 

 4th 3-inch piece 

 5th 3-inch piece 

 6th 3-inch piece 

 7th 3-inch piece 

 8th 3-inch piece 

 24-inch piece . . . 



*Pieces lost off the raft. 



