106 Tzk.'ciity-sci'ciith Annual Meeting 



ing" of the hatching of about lOO eggs, but says nothing, ex- 

 cept in a general way, of feeding and rearing the fry. I have an 

 impression that Mr. Mather has reported his operations at greater 

 length, but I do not remember just when and where his account 

 was published. 



The first real effort in this direction was inaugurated last 

 spring by the United States Fish Commission at a sub-station 

 connected with the Bozeman (Montana) Station, and situated on 

 the inlet (Elk Creek) of Red Rock Lake, the headwaters of the 

 Jefiferson River. This auxiliary station was in charge of Mr. A. 

 |. Sprague, who was detailed from the Leadville Station, and 

 worked under my direction. 



A/Ir. Sprague took some 3,000,000 grayling eggs, 1,000,000 of 

 which were hatched and planted in Elk Creek. Fifty thousand 

 eyed eggs were shipped to the Manchester (Iowa) Station, 50,000 

 to the Leadville (Colorado) Station, and 10.000 to the United 

 States Fish Commission Exhibit at the Omaha Exposition, all of 

 which, by extra precautions in packing, arrived at their destina- 

 tion in good condition. About 1,500,000 were shipped to the 

 Bozeman Station, but many were lost, owing to a lack of ice 

 for packing the eyed eggs. Some green eggs were shipped as an 

 experiment, and though seemingly in good condition on arrival 

 at Bozeman, they all died soon afterward. 



These eggs were shipped over a wagon road some sixty miles 

 in a conmion farm wagon, without springs, and called by cour- 

 tesv "a stage," from Red Rock to Monida, Montana, thence by 

 railroad. The drive of sixty miles is made in one day, by relays of 

 horses, and as the drivers are required to "make time," the eggs 

 were subjected to much jolting. 



The problem of transportation of eyed grayling eggs, how- 

 ever, has been satisfactorily determined. As the period of incuba- 

 tion is so short, it is absolutely necessary that the temperature 

 be kept between 40 degrees and 50 degrees, say at 45 degrees. 

 This can be accomplished by packing ice and dry moss beneath, 

 around and on top of the stack of trays in the egg-case. A good 

 plan, also, is to place an extra ice-hopper, in an inverted position, 

 over the usual hopper; this answers the double purpose of keeping 

 the moss dryer, and also allows more ice to be used on top. It is 

 of the utmost importance that the eggs should not be subjected 

 to the least pressure during transportation. There should be very 

 little, if any, moss placed over the eggs or between the egg-trays. 

 Any pressure on the eggs causes fungus to develop, and is fatal 

 to the life of the embryo. 



