112 Tzventy-ninth Annual Meeting 



thing needed. A brief description of this case may not be amiss: 

 The outside box is 30 inches square, and from 12 to 18 inches 

 deep, according to the numl^er of eggs to be carried. An inner 

 wall of light stulif, say one-half inch thick, of the same depth as the 

 outer box, and 27 inches square, without top or bottom, is pro- 

 vided. The space between the outer and inner walls is packed 

 tightly with dry sphagnum moss or dry sawdust The stack of egg- 

 trays is placed in the center of the box. leaving a space of about 

 five inches between it and the inner wall, which is filled with 

 broken ice. On the top of the trays is a hopper with perpendicular 

 sides, four or five inches deep. The vertical sides of the hopper 

 allow free access to the ice-chamber around the stack of trays. The 

 trays are 12 inches square on the outside, and 1)ut 4-inch deep. 

 This pemiits twice as many trays in a stack as with the ordinary 

 trout trays. The eggs are placed in a single layer on each tray, 

 and covered with a piece of mosquito netting in the usual way. but 

 no moss is placed over it, as grayling eggs will not admit of as 

 much pressure as trout eggs ; the outer membrane of the eggs is 

 quite thin and easily ruptured. By this method of packing, the 

 eggs are received at Bozeman station in as perfect condition as 

 they leave the auxiliary station, after being subjected to a wagon 

 haul of 45 miles and 250 miles by rail, and at a temperature not 

 exceeding 40° F. This has been, also, the condition in which they 

 arrived at distant points, according to reports received, even, as in 

 some cases, when they were en route a week. 



As a matter of experiment, several trays of eggs were shipped 

 but six hours after fertilization to Bozeman station, with the 

 result that fully 25 per cent of the eggs hatched ; heretofore similar 

 experiments with green eggs resulted in the loss of all the eggs 

 before their arrival. By maintaining a temperature not to exceed 

 40° F. the development of the embryo is retarded, and the eggs can 

 be safely shipped to any distance so long as this condition is 

 observed. It is my opinion that grayling eggs can be safely 

 shipped within one or two days after fertilization, but this has yet 

 to be determined by experiment. Shomld this prove to be true,, 

 it will be an important factor where the eggs are to be shipped 

 a long distance, requiring several weeks for the journey. 



