174 



Tzvcnty-ninth Anniial Meeting 



The experiment, however, resulted in what was to the writei 

 a most startHng- discovery. Five lots of one Innidred each, taken 

 from the same lot from which the eggs had been procured for 

 these experiments, showed but 3 2-5 per cent unimpregnated 

 eggs and but few with ruptured yolks, while those subjected to 

 the injury process showed large numbers of what appeared to 

 be unimpregnated, or which looked like those wdiich fish culturists 

 have regarded, when viewed under the microscope, as unmipreg- 

 nated, the disc being' hemispherical, semi-transparent, amber- 

 colored and devoid of all appearance of segmentation. One thing 

 is sure, all of these eggs were dead. 



The following table shows the number ot eggs appearmg 

 normal, that is, Avhere segmentation had taken place, those with 

 ruptured yolks, and those having the appearance of being imim- 

 pregnated : 



It should be here stated that one twin disc was found among 

 the five hundred eggs which had not been submitted to the injur- 

 ing process. 



In another experiment, eight lots of eggs were given ten 

 shakes each Vv^th as uniform force as possible with the right 

 arm. The results were substantially the same as in the case of 

 the above, with the exception that there were rather more rup- 

 tured eggs than in the former case. 



There is obviously but one conclusion to be drawn from the 

 result of these experiments, and that is that the larger portion 



