[Vor. 1 
62 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
one from а middle aged white person (A), the other from a 
colored person (B). 
The samples were collected and treated in à manner similar 
to that outlined in the early part of this paper. In the first 
case (A), transfers were made from all colonies on two plates, 
representing а dilution of one part saliva in ten billion. 
These subcultures, all of cocci, were numbered from 1 to 17 
inclusive. 
In the second case (B), transfers were made from 36 colonies 
which developed on one-third of a plate representing a dilution 
of one part saliva in ten billion. The entire series of cultures, 
numbered from 1 to 36 inclusive, although made from 36 
colonies from a plate containing a total of 100 colonies, were 
found to be made up of coccus organisms. After being incu- 
bated in + 1 nutrient broth for 2 days at 37°C., each of the 
cultures from samples (A) and (B) was transferred to the three 
differential media,—lactose and saccharose broths, and milk. 
The results recorded in tables пт and Iv were observed after 
3 days’ incubation at 37°C. No gas was produced in any of 
the sugar media. A blank determination gave negative re- 
sults throughout on the three media. 
TABLE III 
REACTIONS OF SALIVARY COCCI (A) 
Ё £ Ig Eia 
S % Bl |81%1|4 5 С 3. 
"We в >. 2 . дыз . 
5 12 3 мая S | |$ мясо) $ |$ | Ао ам 
|на на 2 14 
11 ТІНҒІЗІЗТ Cl + pt tit EE SE ELEE, 
ЕЛ, ЛЕЛ, 4+) tit] + 
ft iti ti + УЕ 15 | +i ti ti + 
4 0; 0; 0; O 10 |-Fi - | +] + 16 + +++ 
а ал Ж и ++ 11] ++ 
ЗЕ С; +t + 21+ + ++ 
-Findicates positive reaction. O indicates negative reaction. 
