1914] 
NOLTE—SALIVARY ORGANISMS AND AIR POLLUTION 69 
as favorable places for tests. Hence, a local street car was 
chosen as a source for air samples. The often poorly ventilated 
but well filled motion picture theatres furnished another sup- 
posedly promising sampling place. The third locality chosen 
as a source for air samples was a local 5 and 10 cent store. It 
was thought that this would furnish an ideal source of contami- 
nated air because of the large crowds of people who are con- 
tinually voicing their sentiments and desires. In order to deter- 
mine whether or not the salivary organism is present in an at- 
mosphere which is not in immediate contact with human beings, 
and which is open to the ventilation of nature,the fourth sample 
was taken from the open air. 
DISCUSSION OF THE EXPERIMENTS 
The experiments in table rx are arranged according to the 
dates on which the tests were made. But for convenience in 
this discussion the experiments will be taken up according to 
the source of the samples. 
Experiment 1.—This experiment was carried out primarily 
to test the apparatus. The air sample was taken in a labora- 
tory on the second floor of an old building. There were usually 
at least two people present in the room, and practically no 
ventilation was provided, the doors and windows being con- 
stantly closed. The apparatus used differed from that used in 
the remaining experiments in that two sand filters were used 
in tandem instead of the usual one. During the 15 minutes of 
operation, 7,800 cc. of air were drawn through the sand of both 
filters at the rate of 520 cc. per minute. 
The sand of the first filter was introduced into 15 cc., that of 
the second into 6 ec. of sterile distilled water. Quantities of 
both solutions were plated with the following results: 
TABLE VII 
Filter Plate Quantity Total no. Coccus 
number number plated of colonies colonies 
1 1 166, 2 0 
1 2 106. 2 2 
1 3 2-66. 4 2 
2 4 5 cc. 0 0 
