1914] 
NOLTE—SALIVARY ORGANISMS AND AIR POLLUTION 73 
Experiment 10.—During this experiment, 44 persons were 
seated and 15 standing. It was noted that one transom was 
open. Тһе plate examined (see table rx) gave a total of 12 
colonies, of which 5 were of bacilli and 7 of cocci. Of the latter, 6 
reacted negatively on all three of the differential media, whereas 
1 gave а positive reaction throughout. This makes the fre- 
quency of occurrence of the characteristic salivary coccus form 
1 in 3,600, and, according to table vr, marks the air in this car 
as "questionable" at the time of the experiment. 
Summarizing the car experiments, it is to be noted that in 
three out of five cases the characteristic salivary coccus form 
was isolated, and in such quantity as to mark the air of one 
"unsafe," that of another “probably unsafe," and of а third 
“questionable.” 
EXPERIMENTS 9 AND 11 
These experiments were carried out in a local vaudeville house. 
The construction of the building appeared modern in every 
respect. The lower floor had a seating capacity of about 2,000, 
while the balcony accommodated approximately 1,000 people. 
The house was filled with spectators on the occasions when the 
samples were taken. Upon inquiry, after the surprisingly good 
results given below were obtained, it was found that the build- 
ing was well ventilated by one of the modern appliances for 
this purpose, whereby the volume of air in the building (about 
90,000 cubic feet) was being renewed to a greater or less extent 
every seven-tenths of a minute. For the collection of the air 
samples, the same apparatus was used as in the street car 
experiments, 10,800 cc. of air being drawn through the sand filter 
at the rate of 900 cc. per minute. The sand was introduced into 
15 ce. of sterile distilled water and platings were made as indi- 
cated in table rx. 
Experiment 9.—The air sample was obtained near the center 
of the lower floor of the building about 60 feet from the stage. 
The entire lower floor was packed, and in addition about 100 
or more persons were standing in the rear. On the plate ex- 
amined, a total of 14 colonies developed,—3 mold, 9 bacillus 
and 2 coccus. Molds were very abundant on the other plates. 
One of the coccus forms reacted negatively on lactose and milk 
but positively on saccharose, whereas the other gave negative 
