[Vor. 1 
176 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
bt 
The same as No. 5 in the 1912 experiment. 
The same as No. 1 in the 1912 experiment. 
3. Тһе same as No. 5 in the 1912 experiment, but with 5.26 grams 
of d-glucose (making a glucose solution isotonic жне а 1 per 
cent saccharose solution) added. 
4. The same as No. 1 in the 1912 experiment, but with 5.26 grams 
of d-glucose (making a glucose solution isotonic with a 1 per 
cent saccharose solution) added. 
5. Тһе same as No. 5 in the 1912 experiment, but with 5.32 grams 
of mannite (making a mannite solution isotonie with a 1 per 
cent saccharose solution) added. 
6. The same as No. 1 in the 1912 experiment, but with 5.32 grams 
of mannite (making а mannite solution isotonie with a 1 per 
cent saccharose solution) added. 
7. The same as No. 5 in the 1912 experiment, but with 10.00 
grams of saccharose (making a 1 per cent saccharose solution) 
added. 
8. The same as No. 1 in the 1912 experiment, but with 10.00 
grams of saccharose (making a 1 per cent saccharose solution) 
added. 
nd 
Тһе solutions containing the organie compounds were all made 
isotonie in order to obviate possible differences in growth due to 
different osmotic pressures of the cultural solutions. 
The exact volume of solution necessary to just saturate the 
amount of sand used in each flask was determined and this 
amount of the various solutions added to the corresponding 
flasks. Тһе stoppers were then lightly inserted and one group 
sterilized at a time in a large Kny-Scheerer horizontal autoclav, 
at six pounds pressure for опе and one-quarter hours. 
INOCULATION 
Тһе groups of flasks were transferred directly from the auto- 
clav to the inoculating room which had previously been''steamed 
down." All inoculations were made with a DeVilbis atomizer, 
which, with the exception of the bulb, is made of metal and 
glass throughout. Тһе bulb was removed and the opening of 
the metal tip, to which the former is attached, plugged with 
cotton. After filling the glass container one-half full of solution 
No. 5 (1912 series), the whole (with the exception of the bulb) 
