[Vol. 2 



796 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



same ease as starch, seems very slightly acted upon by the 

 carbohydrases of Ulva. The failure of action on sucrose and 

 lactose is not so surprising as is that on maltose, for one 

 would expect the action on polysaccharides to continue to 

 what is generally held to be directly assimilable sugars, i. e., 

 the hexoses. 



THE CARBOHYDRASES OF ENTEROMORPHA INTESTINALIS 



This series (table iv) was run under exactly the same con- 

 ditions as the one preceding. The " diffusion-extract' ' was 

 from dehydrated tissue about two months old. Ten cc. of this 

 were used with each 50 cc. of substrate, toluene added as an 

 antiseptic, and the flasks kept at a temperature of 35° C. for 

 30 days. 



TABLE IV 



THE ACTION OF A "DIFFUSION-EXTRACT" FROM AIR-DRIED ENTEROMORPHA TISSUE 



UPON CERTAIN CARBOHYDRATES 



Substrate 



Starch . . 

 Dextrin. 

 Glycogen 

 Inulin. . . 



Sugar as glucose in 5 cc. 



nigins. 



S ucrose 

 Lactose 

 Maltose 



9.7 



5.1 



2.8 



Trace 



• t 



• ft 



t ft « • 



30 days 



13.1 

 9.8 



3.9 

 Trace 

 Trace 



t ft f 



• • 



The results for this closely related form are consistent with 

 those obtained for Ulva, the action in the present case, how- 

 ever, being somewhat slower. The more common polysac- 

 charides are acted upon while the disaccharides are not 



attacked. 



THE CARBOHYDRASES OF LAMINARIA AGARDHII 



The water extract from air-dried Laminaria tissue was 

 extremely viscous and upon addition of alcohol, a very heavy 

 precipitate was thrown down that contained a large amount 

 of algal slime. When water was added to this precipitate in 

 the usual ratio a very viscous diffusion was obtained. Ten 

 cc. of the ' ' diffusion-extract ' ' were used with 50 cc. of the sub- 

 strate and 2 per cent toluene added as an antiseptic. The 

 flasks were kept at a temperature of 20-22° C. for 100 days, 



