[Vol. 2 



810 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



nel to which a drawn-out piece of glass tubing had been at- 

 tached, into about 100 cc. of cold distilled water, the water 

 being stirred constantly while the olive oil was being run in. 

 A milk-white emulsion made up of extremely small suspended 

 globules of oil resulted. In an emulsion carefully made, most 

 of these globules are small enough to show Brownian move- 

 ment. The alcohol was driven off finally by heating and the 

 emulsion made up to the desired concentration. 



Both emulsions stand up well. In the latter, however, there 

 is a tendency toward flocking out by some of the smaller par- 

 ticles upon the addition of any salt-containing substance, such 

 as, for instance, algal powder; but, on the other hand, it has 

 the advantage of being more easily checked up because of its 

 simpler composition. 



TABLE XI 



LIPOLYTIC ACTION OF THE SEVERAL ALGAE UPON OLIVE OIL-CASEIN EMULSION 





Nui 



ruber cc. of N/10 NaOH to neutralize 10 cc. of substrate 





4 days 



10 days 



15 days 



Alga 



Emulsion 

 + tissue 



Emulsion 



* 



Water 

 + tissue 



: Net 



: acidity 



!"* Emulsion 

 ^ + tissue 



c 

 .2 



"35 



.25 

 2 



3 



u tn 



.i 



.i 

 .i 



1.0 



.05 



.05 



.05 



.1 



.15 



.05 



T3 



.95 



.95 



.8 



.45 



.35 

 1.8 



.25 

 1.5 



.55 



.00 



Emulsion 

 + tissue 



Emulsion 



Water 

 + tissue 



Net 

 acidity 



Viva 

















1 ?S 











Mesoploea 



.6 



.00 



.1 



.5 



1 . 00 

 1.50 



.4 

 1.85 



.3 

 1.6 



.9 



.35 



.1 



.05 



.00 



.00 



.00 



.00 



.2 



.3 



2.2 



.6 



.075 



1.525 



Ascobhvllutn 



^m m ^-^ *™ ^f 













.6 



2.3 



.1 



• • m m 



.025 

 .1 



.475 



Chondrus 



1.2 



.00 



.05 



1.15 



2.200 



A vatdhiella 



^^^ V ^^^^ ^**^ ^^ 



Ceramiutft 



















Rhodymenia 



Champia 



.3 



-2 



.1 



.1 



.05 

 .05 



.15 



.05 



















In all the lipolytic experiments, algal powder or fresh algal 

 tissue crushed with fine quartz sand, was used as a source of 

 enzyme action. In some of the original series the olive oil- 

 casein emulsion was employed, but on account of the danger 

 arising from a possible hydrolysis of the casein with a re- 

 sulting increase in acidity, the alcohol emulsion was used in 

 the later work. 



Lipolytic action of the several algae upon olive oil-casein 

 emulsion. — In this experimental series (table xi) flasks were 

 set up containing 50 cc. of olive oil-casein emulsion as a sub- 



