1915] 



DAVIS ENZYME ACTION IN MARINE ALGAE 



811 



strate, 5 grams of crushed algal tissue for enzyme action, and 

 10 cc. of 95 per cent alcohol as an antiseptic. Checks were 

 employed wherein the flasks in one case contained the emul- 

 sion alone, and in another case, the same weight of algal pulp 

 in distilled water. The flasks were maintained for 15 days 

 except for the forms especially noted. At intervals 10 cc. por- 

 tions were removed and titrated against N/10 NaOH with 

 phenylphthalein as an indicator. 



Lipolytic action on alcohol-water-olive oil emulsion. — Be- 

 cause of the possibility of the hydrolysis of the casein in the 

 emulsion used in the preceding experiments 

 xii ) employing the alcohol-water emulsion ^ 

 check. 



a series (table 



r as set up as a 



This emulsion alone was practically neutral but a 



TABLE XII 



ACTION OF THE SEVERAL ALGAE UPON ALCOHOL-WATER-OLIVE-OIL 



EMULSION 



Alga 



Number cc. N/10 NaOH to neutralize 10 cc. 



substrate after 10 days 



Ulva 



Enteromorpha 



Mesogloea 



A scophyllum . 

 Laminaria . . . 

 Chondrus .... 

 Agardhiella . . 

 Ceramium . . . 



Rhodymenia 

 Champia 



Emulsion 

 + tissue 



.9 



.8 



.65 



.2 

 1.15 

 1.45 



.25 



.85 



.525 



.125 



Emulsion 

 alone 



.00 

 .00 

 .00 

 .00 

 .00 

 .00 

 .00 

 .00 

 .00 

 .00 



Water + 

 boiled tissue 





.05 



.025 



.05 



.15 



.02 



.1 



.05 



.1 



.05 



.15 



Water + 

 tissue 



.05 

 .1 



.075 

 .15 



.3 



Net 

 acidity 



.85 

 .7 



.575 



.05 



.85 



.35 



1.10 



.05 



.20 



.20 



.65 



.15 



.375 



.15 



.00 



slight acidity was produced by the addition of the algal 

 powder. A negligible amount of the oil globules ran together 

 and collected at the surface of the liquid after some days, but 

 the bulk of the emulsion stood up well. As in the preceding 

 series, 5 grams of the fresh tissue were used as a source of 

 lipolytic activity, and the alcohol in which the olive oil had 

 been dissolved served as an antiseptic. Fifty cc. of the emul- 

 sion were used as a substrate, and the flasks maintained at a 



temperature of 

 Lipolytic acti 



o 



C. for 10 days 



c action on triacetin. — The lipolytic activity of dry 

 >wder of Ulva, Mesogloea, and Chondrus was tested, 

 5 per cent solution of triacetin as a substrate. Two 



