[Vol. 2 



812 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



ams of the tissue powder 



used, otherwise the 



(table 



ged exactly as the preceding and kept 



at room temperature for 25 day 



Action 



A series was set up with methyl 



acetate, ethyl acetate, and ethyl butyrate in .25 per cent solu 

 tion, using 2 grams of algal powder with 50 cc. of the sub 



TABLE XIII 



ALGAE 





Number cc. of N/10 



10 cc. subs 



NaOH t 



strate aft 



o neutra 



er 



lize 





Alga 



10 days 



25 days 



Tri- 

 acetin 

 + tissue 



Water + 

 tissue 



Tri- 1 

 acetin 

 alone 



Net 

 acidity 



Tri- 



acetin + 

 tissue 



Water + 

 tissue 



Tri- 

 acetin 

 alone 



Net 

 acidity 



Ulva 



.3 



.025 



.05 



.35 



.1 

 .1 

 .1 



.175 



.1 



.1 



.5 

 .4 

 .8 



.15 

 .2 



.4 



.15 

 .15 

 .15 



.2 



Mesogloea 



Chondrus 



.25 

 .55 



.05 



.25 



strate in 20 per cent alcohol. Titrations 



made from 



time to time against N/10 NaOH with phenylphthalein 

 indicator. Even after 60 days at room temperature 



m- 



acidity was observable over the checl 



General results f 



The 



serve to show that, although slight, there is distinct lipolytic 

 activity in most of the forms investigated. The various 

 groups of algae are not so distinct regarding this activity as 

 was the case with the carbohydrases, nor does the activity of 

 the individual alga in this case relate itself particularly to the 



action. Agard- 



activity shown by the form in its carbohydrase action. 

 hiella hydrolyses the polysaccharides more rapidly than an} 

 other alga, yet its lipolytic activity is very low. Likewise 

 Laminaria. so inactive in the previous group of enzymes, it 



s 



most active on fats. Fucus, on the other hand 



found in previous work to ha\ 

 hydrates or fats. 



action on either carb 



The action is especially evidenced by use of the 



emulsion. In general 



alcohol-water emul 



difference prob 



