1916] 
GILMAN——CABBAGE YELLOWS 20 
transferred to other Van Tieghem cells by means of a sterile 
pipette. They were immediately placed in the incubators at 
the desired temperatures, and observed at intervals for ger- 
mination. All observations were made in duplicate, and the 
trials were repeated twice to verify them. Тһе temperatures 
used were 8-10°C., 10-12°C., 16°C., 21°C., and 33°C. The 
results are brought together in table 1. 
TABLE I 
GERMINATION OF CONIDIA OF FUSARIUM Aerer ново AT VARIOUS 
TEMPERATURE 
Germination (+) at the following temperatures 
Hours 
of 8-10? C. 10-12? C. 1626. 212; е С. 
exposure 
Trial no. Trial no. Trial no. Trial no. Trial no. 
1 2 1 2 1 2 1 2 1 2 
I ee — — БЕН IH == = = = =з а 
Да Жы. -- — -= E ща PS Es E: zz - 
Hi Mor — — — == ши = = aia 4 4 
Dro АГ — — — — — = 7 ВЕЕ + + 
NEC — — — 7 +* T* aR E + + 
Lae -- -- -- — + + + + + + 
BE... = — — — + + + + + “Б 
Ee — -- — | —t + + + 4- ch mE 
ШЕ». — — — 4 + + A 4- + 
ater test in which more frequent observations were made, conidia at 
this Senaye (16°C.) germinated 12 hours after the beginning of the 
exposure. 
ł At this temperature (10-12° C.) spores were found to germinate after 36 
n a later experiment. The number that germinated, however, was very 
small and the growth eec Zi slow 
It will be noted that, as was to be expected, spores of the 
fungus germinated best at the higher temperatures of the 
experiment, although they were able to grow slowly at the 
lower temperatures. These facts are further borne out by the 
growth of the fungus on potato agar. Transfers of a bit of 
the mycelium from a rapidly growing culture were placed in 
the center of plates of potato hard agar, and the plates were 
then placed in the incubators at the desired temperatures. 
3 
