[VoL. 5 
38 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
TABLE III 
RESULTS OF INOCULATIONS WITH PURE CULTURES OF FUSARIUM 
CONGLUTINANS 
Plants per pot 
Pot 
no, Kind of soil Treatment of soil Percent- 
Total No. age 
no. diseased | diseased 
1 | Infected.......... беегіпгей................. 7 0 0 
2 | Infected........... Sterilized oii Ve RN oo be Ü. ondas d 
3 | Infected ter'z'd and inoc'l'd........ 4 3 75 
4 | Infected........... Ster'z'd and inoc’l’d........ 1 1 100 
5 | Infected........... Монша tics cai Lun 4 80 
6 | Infected........... Normál i. Ae Bee aer carne 6 6 100 
7 | Uninfected........ МОКа beck eius 0 0 
8 | Uninfected........ Мота ooh vvv 1 PEREP ETT 
9 | Uninfected........ Ster'z'd and inoc’l’d........ 3 1 33 
10 | Uninfected. ....... Ster'z'd and inoc'l'd........ 5 5 100 
11 | Uninfected........ Ster'z'd and inoc'l'd........ 1 1 100 
12 | Uninfected........ Ster'z'd and inoc'l'd........ u 1 50 
13 | Uninfected........ Sterilized................. 6 0 0 
14 | Uninfected........ Sterilized. оо: 4 0 0 
15 | Uninfected........ Ster'z'd and infect. leaves... 2 1 50 
16 | Uninfected........ Ster'z'd and infect. leaves... O VT ays Ee 
The results are not as conclusive as they might be, how- 
ever, as the number of plants used was very small due to the 
poor seed mentioned above. The pots in which no plants 
grew in either of the duplicates were omitted. An idea of 
the appearance of the plants at the time of making counts 
may be had from pl. 1, fig. 2. It should be noted that these 
successful inoculations were made at the warmest time in the 
summer. Plates were made on July 14 from plants in each 
pot, and in all cases the diseased seedlings gave pure cultures 
of F. conglutinans, while those from the controls remained 
sterile. Culture X was one of these and upon reinoculation 
again produced the disease (table v). 
On June 12 the above experiment was repeated, using three 
flats of soil (from the experimental field), two of which had 
been sterilized at eleven pounds pressure for four hours on 
two successive days, the other remaining untreated. One of 
