` [Vor. 3 
74 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
plants showed wilting in nine days but the others all remained 
upright and turgid, having completely recovered. There was 
no discoloration of the leaves in connection with the wilting. 
In a later experiment with older plants, the entire stem 
of each plant was killed for a distance of 3 em. from the surface 
of the ground by allowing it to stand in alcohol for three 
minutes. After nine days, wilting appeared in one of the 
five plants, the lowest leaves drooping first, but with no dis- 
coloration such as occurs under the influence of fungous at- 
tack, nor falling of the wilted leaves. A second plant suc- 
cumbed on the twelfth day, but again there was rapid wilting 
with no loss of green coloring matter. By the eighteenth day 
all the plants had wilted, but even where the injury had been 
least and the wilting slowest there was no discoloration or 
falling of the leaves. The experiment was repeated with older 
plants in March, 1915, with similar results. The wilting al- 
ways took place without discoloration of the leaves, nor did 
any of them drop before the entire head was wilted. 
Further work was started, therefore, to see whether the fun- 
gus could produce in pure culture any substances toxic to eab- 
bage. For this study two Erlenmeyer flasks of half-liter ca- 
pacity, each containing 100 сс. of Uschinsky’s fluid, were in- 
oculated with a virulent culture of F. conglutinans. After two 
weeks the fungus-felt was filtered from the solution by means 
of a pressure filter, and the solution, after dilution to 500 ce., 
was poured in two glass tumblers, in which germinated eab- 
bage seedlings were then placed. Controls of Uschinsky's 
fluid diluted 2:5, tap water, and Pfeffer's full nutrient were 
used in connection with the experiment. Difficulty was ex- 
perieneed in getting the plants to start because of the desic- 
cation of the young cotyledons. By placing the plants in an 
incubator under humid conditions, the plants growing on tap 
water and Pfeffer’s solution grew fairly well, but on Uschin- 
sky’s fluid, neither on that in which the fungus had been grow- 
ing nor on the sterile fluid, was it possible to get any growth, 
indicating that some other media or methods will have to be 
used. Further work on this point is being pursued. 
