[Vor. 3 
262 ANNALS OF THE Missourr BOTANICAL GARDEN 
from diseased tissues, and the results of inoculation experi- 
ments on animals. 
The presence of cryptococci in tumors has been mentioned 
by a large number of authors, but the tumors thus parasitic 
do not all pertain to the type of malignant tumors. Binaghi 
(796) investigated fifty-three cases of epithelioma and iso- 
lated parasitic organisms in forty instances. The failure to 
obtain parasites in the remaining thirteen, according to this 
author, may be due to the fact that the part examined was 
either in an early stage of development or not infected. 
These organisms, identical with eryptococci in their mor- 
phological and physiological characters, were not found in 
other pathological or normal tissue. Consequently, they were 
considered as the specific cause of epithelioma. Maffucci and 
Sirleo (798) obtained ten or more cultures of yeasts from 
the thirty-nine tumors which they examined. Only one was 
pathogenie for guinea-pigs, and in these animals it produced 
fibrinous pneumonitis and abscesses under the skin or in the 
kidneys. The results indieated that a new formation of 
sarcoma tissue did not take place. КопсаП, Corselli, and 
Plimmer obtained cultures of fungi from malignant tumors, 
but only in a few cases were they pathogenic for animals. 
Leopold (700) reported having isolated pure cultures of сгур- 
іюсоссі from over 80 рег cent of the non-uleerated tumors 
investigated, the cultures having been obtained from the 
center of the diseased tissue. 
Loeb, Moore, and Fleisher (713) were unable to confirm 
the results of Leopold. Of the seventeen tumors examined, 
only one gave a culture of a yeast-like organism. In no case 
of human cancer has the causative significance of a microor- 
ganism so far been proved. Moreover, Busse (’03) was never 
able to obtain a single culture of Cryptococcus from non-ul- 
cerated tumors. 
No one has been able to demonstrate the development of 
tumors histologically comparable to cases of sarcoma, by 
the inoculation experiments on animals with cultures of 
Cryptococcus. By inoculation of C. tumefaciens in the white 
rat, Curtis obtained results in which ‘‘la tumeur etait iden- 
