[Vor. 3 
276 ANNALS OF THE Missovumi BOTANICAL GARDEN 
red cultures of T'orula. It liquefies gelatin rapidly, but culture 
2 liquefies gelatin very slowly, and culture 3 not at all. Growth 
takes place by budding, as in the true yeasts. The cells (fig. 
1) are oval or spherical, varying in size from 3-4 X 4-би. 
In sugar nutrient solutions the organism develops rapidly, 
with the formation of an abundant sediment of yeast cells; 
but the film, if any, is very thin and made up of colonies 
loosely connected. In acid yeast-water solution a yellow- 
brown sediment is formed except in the presence of malic 
acid in which ease the red pigment has practically disap- 
peared, the sediment being almost white. No spores are 
formed on moist porous plates or on Gorodkowa’s test 
medium, although in many cells there appeared two to four 
fat globules that are much like endospores in appearance. 
Culture 2, Torula sp?—The cells of this strain (fig. 2) vary 
in size from 3-3.5 X 4-6». Growth is by budding from all 
sides of the mother 
eell and without 
the formation of 
mycelium. Devel- 
opment at 37°C. 
is more rapid than 
at room tempera- 
ture. Cultures 
grown in yeast wa- 
Fig. 9. Culture 9. Cellsx 2000. ter with the addi- 
tion of saccharose, glucose, levulose, maltose, and lactose, 
produce a heavy red deposit of yeast cells and a slight ring 
formation on the surface of the liquid. In yeast water con- 
taining 1 per cent of acids—citrie, malic, tartaric, and succinic 
acids—development is as good as in the sugar nutrient solu- 
tions. On yeast-water agar the colonies are very small, 
elliptical or oval in outline, and .2 mm. in diameter. Gelatin 
is slowly liquefied. No spores are formed. 
Culture 3, Torula sp?—This organism, obtained from the 
nectar of Salvia splendens, is brownish red in color. The cells 
