[Vor. 3 
278 ANNALS OF THE MissoURI BOTANICAL GARDEN 
malic acids the separate colonies are connected into a net- 
like film. Gelatin is not liquefied, and no spores are formed. 
Culture 5, Torula sp?—The cells of this species (fig. 5), ob- 
tained from the nectar of Oenothera sp?, are oval to elliptical, 
2-2.5 >x 45. On 
beer-wort agar and 
gelatin the colonies 
are small, white, 
and spherical. In 
sugar nutrient so- 
lutions and in yeast 
water containing 
citric, malic, or lac- 
Fig. 5. Culture 5. Cells 2000. tic acids, there is 
a considerable sediment of yeast cells and a thin film on 
the surface of the culture liquid, In yeast water containing 
tartaric acid the colonies remain more or less distinctly 
formed on the bottom of the culture flask. No spores are 
produced, and gelatin is not liquefied. 
Culture 6, Torula sp?—The colonies of this species of 
Torula, obtained from the seeds of Zea Mays, may be dis- 
tinguished from cultures 
4 and 5 by the growth 
appearance on agar and 
gelatin. The margin of 
the colonies is irregular 
and extends radially from % 
the center of growth. The Се 
cells (fig. 6) are smaller, 
being 1.5-2.5 X 2-3.5y. 
Multiplieation is by bud- 
ding, more particularly at 3 
the ends of the mother 
cells. In nutrient solutions Fig. 6. Culture 6. Cellsx 2000. 
containing galaetose and levulose, development takes place 
rapidly, with the ^ "mation of a sediment of yeast cells and a 
very dense wrink.. ` film on the surface of the liquid. In 
nutrient solutions containing maltose and lactose, the film is 
