19161 
Emic—Yerast Funct 283 
are crowded, only yeast cells are formed. Saccharose and 
maltose are fermented. In yeast water containing citric, 
tartaric, or succinic acids, the sediment is made up of yeast 
cells, whereas the black, leathery film is composed of mycelia 
and chlamydospores. The cells (fig. 11) vary in size from 
4-6 X 10-12» or 2 x 10-504. The many-celled muriform 
spores are found in cultures grown in acid yeast-water solu- 
tions. 
Culture 12, Alternaria sp?—This fungus is differentiated 
in part from the other black yeast-like fungi by the late ap- 
Fig. 11. Culture 11. Vegetative cells, chlamydospores, and muriform spores. 
pearance of chlamydospores and the predominance of yeast 
cells. In early cultures on agar the colonies are yeast-like 
in appearance, and the mycelium starts to develop only 
after the medium has become less moist. In nutrient solu- 
tions containing sugars the growth is very rapid, and a 
ring of colonies appears on the surface. In yeast water 
containing organic acids there is a sediment of yeast cells, 
or the growth is limited to isolated colonies on the bottom 
of the flask. The ring appearing on the surface of the liquid 
is white in color except in the presence of lactic acid, in 
which case it is black. This fungus, a species of Alternaria, 
has dark chlamydospores and many-celled muriform spores. 
The cells (fig. 12) vary in size from 3-4 X 9-60и. Gelatin is 
